Faculty Publications

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Author: search.filters.author.Iyyaswami, R.Subject: search.filters.subject.Aqueous two phase system

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Now showing 1 - 6 of 6
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    Recovery and Partial Purification of Bovine ?-Lactalbumin from Whey Using PEG 1000 – Trisdoium Citrate Systems
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Sivakumar, K.; Iyyaswami, R.
    The extraction of ?-Lactalbumin (?-La) and ?-Lactoglobulin (?-Lg) from whey have been carried out in the Aqueous Two Phase System (ATPS) composed of PEG 1000 – tri sodium citrate. The suitable conditions for the simultaneous partitioning of ?-La into the top phase and ?-Lg in bottom phases was identified using the pure ?-La and ?-Lg. Maximum partition coefficient (k) of 16.67 was achieved for ?-La at the system condition of 28% (w/w) PEG 1000-14% (w/w) tri sodium citrate at pH 8 and 40°C, whereas in this condition k of ?-Lg is 0.27. 30% (w/w) whey loaded to this system yielded the maximum recovery of ?-La and ?-Lg. © , Copyright © Taylor & Francis Group, LLC.
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    Synergistic extraction of ?-Lactalbumin and ?-Lactoglobulin from acid whey using aqueous biphasic system: Process evaluation and optimization
    (Elsevier, 2015) Kalaivani, S.; Iyyaswami, R.
    The present investigation focused on the simultaneous partitioning of ?-Lactalbumin (?-La) to the PEG rich phase and ?-Lactoglobulin (?-Lg) to the salt rich bottom phase from acid whey using polyethylene glycol 1000-trisodium citrate systems. The effect of Tie Line Length (TLL), pH, volume ratio and whey concentration on the proteins partitioning was evaluated. Further the Response Surface Methodology (RSM) was employed to analyze the synergistic and interactive effect of these variables (TLL, pH and whey loading) on the responses (recovery and purity of ?-La and ?-Lg). Optimum values of these variables were obtained for different goals through desirability based multi response optimization. A system of 54.12% (w/w) TLL with 30.6% (w/w) whey at pH 7.23 separates ?-La with 89% recovery and purity of 96% in the top phase, whereas that of ?-Lg in the bottom phase was 96% and 76% respectively. © 2015 Elsevier B.V.
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    Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Bolar, S.; Iyyaswami, R.; Belur, P.D.
    L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
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    Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation
    (Springer India sanjiv.goswami@springer.co.in, 2015) Nagaraja, V.H.; Iyyaswami, R.
    A suitable Aqueous Two Phase System (ATPS) has been identified for the partitioning of crude fish proteins from fish processing industrial effluent. A detailed study has been performed to analyze the influence of various parameters on protein partitioning such as molecular weight of PEG, effect of different salts (MgSO4, K2HPO4, Na3C6H5O7, Na2SO4, (NH4) 2PO4, K3C6H5O7) and their concentrations, pH, temperature, Tie Line Length (TLL), effluent loading and volume ratio. PEG 2000 - sodium sulphate ATPS was found to be a most favourable system among the selected ATPS for higher partition coefficient of proteins. The binodal curve and equilibrium characteristics of PEG 2000 - sodium sulphate were established and fitted to empirical equations. The equilibrium compositions (tie line) were correlated using Othmer–Tobias and Bancroft equations. © 2014, Association of Food Scientists & Technologists (India).
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    Aqueous two phase based selective extraction of mannose/glucose specific lectin from Indian cultivar of Pisum sativum seed
    (Elsevier B.V., 2019) Rashmi, B.S.; Iyyaswami, R.
    Pisum sativum lectin (Psl) being a high-value protein has marked its application in the biomedical and therapeutic field. Aqueous two phase extraction (ATPE) was implemented as a selective partitioning technique for the partial purification of Psl from its seeds. PEG/citrate based biodegradable aqueous two phase system (ATPS) was screened and the factors such as the type and concentration of citrate salts, molar mass and concentration of polyethylene glycol (PEG), tie line length (TLL) and additive (NaCl) concentration, pH, crude load and volume ratio were studied for the selective partition of Psl. The Psl was successfully extracted to the top phase in the ATPS formed with 18% PEG 6000/16% sodium citrate at 41.01% TLL, 2% NaCl and pH of 7.5. A volume ratio of 0.76 and a crude load of 20% showed maximum activity yield of 122.12% with the purification factor of 16.26. The subunits of Psl namely ? and ? were identified with a molecular weight of 6 and 18 kDa respectively during the purity analysis using SDS PAGE and HPLC. © 2019 Elsevier B.V.
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    Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization
    (Elsevier B.V., 2020) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS. © 2020 Elsevier B.V.