Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
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Date
2015
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Publisher
Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106
Abstract
L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
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Keywords
Solvent extraction, Surface properties, Aqueous two phase system, Glutaminase, Neutral salts, Optimal conditions, Partition coefficient, Purification factors, Response surface methodology, Volume ratio, Purification
Citation
Separation Science and Technology, 2015, 50, 9, pp. 1360-1368