Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System

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dc.contributor.authorBolar, S.
dc.contributor.authorIyyaswami, R.
dc.contributor.authorBelur, P.D.
dc.date.accessioned2026-02-05T09:33:43Z
dc.date.issued2015
dc.description.abstractL-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
dc.identifier.citationSeparation Science and Technology, 2015, 50, 9, pp. 1360-1368
dc.identifier.issn1496395
dc.identifier.urihttps://doi.org/10.1080/01496395.2014.967410
dc.identifier.urihttps://idr.nitk.ac.in/handle/123456789/26275
dc.publisherTaylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106
dc.subjectSolvent extraction
dc.subjectSurface properties
dc.subjectAqueous two phase system
dc.subjectGlutaminase
dc.subjectNeutral salts
dc.subjectOptimal conditions
dc.subjectPartition coefficient
dc.subjectPurification factors
dc.subjectResponse surface methodology
dc.subjectVolume ratio
dc.subjectPurification
dc.titlePurification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System

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