Recovery and Partial Purification of Bovine ?-Lactalbumin from Whey Using PEG 1000 – Trisdoium Citrate Systems
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Date
2015
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Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106
Abstract
The extraction of ?-Lactalbumin (?-La) and ?-Lactoglobulin (?-Lg) from whey have been carried out in the Aqueous Two Phase System (ATPS) composed of PEG 1000 – tri sodium citrate. The suitable conditions for the simultaneous partitioning of ?-La into the top phase and ?-Lg in bottom phases was identified using the pure ?-La and ?-Lg. Maximum partition coefficient (k) of 16.67 was achieved for ?-La at the system condition of 28% (w/w) PEG 1000-14% (w/w) tri sodium citrate at pH 8 and 40°C, whereas in this condition k of ?-Lg is 0.27. 30% (w/w) whey loaded to this system yielded the maximum recovery of ?-La and ?-Lg. © , Copyright © Taylor & Francis Group, LLC.
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Keywords
Purification, Solvent extraction, Aqueous two phase system, Beta-lactoglobulin, Partial purification, Partition coefficient, Suitable conditions, System conditions, Tri-sodium citrates, whey, Sodium
Citation
Separation Science and Technology, 2015, 50, 6, pp. 833-840