Recovery and Partial Purification of Bovine ?-Lactalbumin from Whey Using PEG 1000 – Trisdoium Citrate Systems

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dc.contributor.authorSivakumar, K.
dc.contributor.authorIyyaswami, R.
dc.date.accessioned2026-02-05T09:33:50Z
dc.date.issued2015
dc.description.abstractThe extraction of ?-Lactalbumin (?-La) and ?-Lactoglobulin (?-Lg) from whey have been carried out in the Aqueous Two Phase System (ATPS) composed of PEG 1000 – tri sodium citrate. The suitable conditions for the simultaneous partitioning of ?-La into the top phase and ?-Lg in bottom phases was identified using the pure ?-La and ?-Lg. Maximum partition coefficient (k) of 16.67 was achieved for ?-La at the system condition of 28% (w/w) PEG 1000-14% (w/w) tri sodium citrate at pH 8 and 40°C, whereas in this condition k of ?-Lg is 0.27. 30% (w/w) whey loaded to this system yielded the maximum recovery of ?-La and ?-Lg. © , Copyright © Taylor & Francis Group, LLC.
dc.identifier.citationSeparation Science and Technology, 2015, 50, 6, pp. 833-840
dc.identifier.issn1496395
dc.identifier.urihttps://doi.org/10.1080/01496395.2014.964729
dc.identifier.urihttps://idr.nitk.ac.in/handle/123456789/26311
dc.publisherTaylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106
dc.subjectPurification
dc.subjectSolvent extraction
dc.subjectAqueous two phase system
dc.subjectBeta-lactoglobulin
dc.subjectPartial purification
dc.subjectPartition coefficient
dc.subjectSuitable conditions
dc.subjectSystem conditions
dc.subjectTri-sodium citrates
dc.subjectwhey
dc.subjectSodium
dc.titleRecovery and Partial Purification of Bovine ?-Lactalbumin from Whey Using PEG 1000 – Trisdoium Citrate Systems

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