Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation
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Date
2015
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Publisher
Springer India sanjiv.goswami@springer.co.in
Abstract
A suitable Aqueous Two Phase System (ATPS) has been identified for the partitioning of crude fish proteins from fish processing industrial effluent. A detailed study has been performed to analyze the influence of various parameters on protein partitioning such as molecular weight of PEG, effect of different salts (MgSO4<inf>,</inf> K<inf>2</inf>HPO<inf>4,</inf> Na<inf>3</inf>C<inf>6</inf>H<inf>5</inf>O<inf>7,</inf> Na<inf>2</inf>SO<inf>4,</inf> (NH<inf>4</inf>) <inf>2</inf>PO<inf>4,</inf> K<inf>3</inf>C<inf>6</inf>H<inf>5</inf>O<inf>7</inf>) and their concentrations, pH, temperature, Tie Line Length (TLL), effluent loading and volume ratio. PEG 2000 - sodium sulphate ATPS was found to be a most favourable system among the selected ATPS for higher partition coefficient of proteins. The binodal curve and equilibrium characteristics of PEG 2000 - sodium sulphate were established and fitted to empirical equations. The equilibrium compositions (tie line) were correlated using Othmer–Tobias and Bancroft equations. © 2014, Association of Food Scientists & Technologists (India).
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Keywords
Effluents, Fish, Phase diagrams, Sewage, Solvent extraction, Aqueous two phase system, Bancroft equation, Empirical equations, Equilibrium compositions, Industrial effluent, Partition coefficient, Partitioning coefficients, Protein partitioning, Proteins
Citation
Journal of Food Science and Technology, 2015, 52, 6, pp. 3539-3548