Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation

Abstract

A suitable Aqueous Two Phase System (ATPS) has been identified for the partitioning of crude fish proteins from fish processing industrial effluent. A detailed study has been performed to analyze the influence of various parameters on protein partitioning such as molecular weight of PEG, effect of different salts (MgSO4<inf>,</inf> K<inf>2</inf>HPO<inf>4,</inf> Na<inf>3</inf>C<inf>6</inf>H<inf>5</inf>O<inf>7,</inf> Na<inf>2</inf>SO<inf>4,</inf> (NH<inf>4</inf>) <inf>2</inf>PO<inf>4,</inf> K<inf>3</inf>C<inf>6</inf>H<inf>5</inf>O<inf>7</inf>) and their concentrations, pH, temperature, Tie Line Length (TLL), effluent loading and volume ratio. PEG 2000 - sodium sulphate ATPS was found to be a most favourable system among the selected ATPS for higher partition coefficient of proteins. The binodal curve and equilibrium characteristics of PEG 2000 - sodium sulphate were established and fitted to empirical equations. The equilibrium compositions (tie line) were correlated using Othmer–Tobias and Bancroft equations. © 2014, Association of Food Scientists & Technologists (India).