Browsing by Author "Pooja, N."
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Item Assessment of biocompatibility for citric acid crosslinked starch elastomeric films in cell culture applications(Nature Research, 2025) Pooja, N.; Ahmed, N.Y.; Mal, S.S.; Bharath, P.A.S.; Zhuo, G.-Y.; Noothalapati, H.; Managuli, V.; Mazumder, N.This study investigates the synthesis of potato starch elastomers reinforced with silicon dioxide (SiO2) and citric acid as a crosslinking agent to enhance their mechanical and barrier properties. Surface morphology analysis using optical microscopy revealed that pure potato starch films had uneven surfaces. However, higher SiO2 concentrations increased roughness, while citric acid crosslinked films displayed smoother surfaces overall. Water vapor transmission rates (WVTR) indicated that native starch films were highly hydrophilic, while SiO2 incorporation and citric acid crosslinking significantly reduced WVTR of 17% (30% lower than native film), enhancing the barrier properties. Tensile strength testing revealed that citric acid crosslinking increased the tensile strength by 25%, while SiO2 further reinforced the films but decreased elasticity by 15%. SiO2 had little impact on degradation rates, while citric acid crosslinking delayed microbial growth, extending film longevity by 20%. Biocompatibility assays using SiHa, HT-29, and HEK 293 cell lines revealed that the films had varying degrees of cell confluency. Films with both SiO2 and citric acid showed improved confluency (20% higher) compared to films containing only SiO2. However, citric acid alone resulted in the highest confluency (95% viability), suggesting its significant role in biocompatibility. This eco-friendly approach demonstrates substantial advancements in film properties, offering potential applications in diverse biomedical industries. © The Author(s) 2025.Item Biocompatiblity of potato starch-based elastomer films for cell culture(Optical Society of America, 2023) Ahmed, N.Y.; Pooja, N.; Mal, S.S.; Mazumder, N.Novel biodegradable elastomers based on potato starch are synthesized and utilized as cell substrates. The elastomers were characterized in terms of their functional properties. To assess the suitability of th e synthesized elastomers for biomedical applications, studies on cell adhesion and cell viability were carried out. © Optica Publishing Group 2023, © 2023 The Author(s)Item Comparative analysis of biopolymer films derived from corn and potato starch with insights into morphological, structural and thermal properties(Springer Nature, 2024) Pooja, N.; Banik, S.; Chakraborty, I.; Sudeeksha, H.C.; Mal, S.S.; Srisungsitthisunti, P.; Patil, A.; Mahato, K.K.; Mazumder, N.Starch biopolymer films were prepared using the solvent casting method involving acetic acid hydrolysis and glycerol plasticization. This process facilitated a more uniform distribution of plasticizers within the starch matrix, enhancing the films' flexibility. Fourier-transform infrared (FTIR) and Raman spectroscopy confirmed the formation of ester linkages and structural changes in the biopolymer films, attributed to glycerol integration. The optimal formulation comprised 6% starch, 6.8% acetic acid, and 6.8% glycerol. X-ray diffraction (XRD) analysis revealed a reduction in crystallinity of the starch during film formation, enhancing flexibility. Second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy indicated that potato starch films had higher crystallinity compared to corn starch films. Thermal analysis via differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) showed that potato starch films exhibited lower gelatinization temperatures and higher thermal stability compared to corn starch films. Functional characterization demonstrated that higher starch content decreased water solubility and water vapor transmission rate, while increasing starch content improved the film's structural integrity. The films were hydrophilic, with static water contact angles indicating moderate wettability. Degradation studies showed that the films were stable in neutral and basic conditions but degraded under acidic conditions over time. The results suggest that potato starch films, with optimized glycerol and acetic acid content, offer improved flexibility, thermal stability, and structural integrity compared to corn starch films. Their performance in various conditions highlights their potential for specific applications, particularly where moisture and environmental stability are critical. © The Author(s) 2024.Item Evaluation of physicochemical properties of citric acid crosslinked starch elastomers reinforced with silicon dioxide(Royal Society of Chemistry, 2024) Pooja, N.; Chakraborty, I.; Mal, S.S.; Bharath Prasad, A.S.; Mahato, K.K.; Mazumder, N.Thermoplastic starch (TPS), derived from renewable resources, offers advantages such as biodegradability and lower production costs compared to petroleum-based plastics. However, its limited mechanical properties pose a challenge for broader applications. This research aims to explore the potential of enhancing the mechanical and barrier properties of TPS films through the incorporation of silicon dioxide as a reinforcement filler and citric acid as a crosslinking agent. By introducing silicon dioxide as a reinforcement filler, the mechanical strength of the TPS films is expected to be improved. Additionally, the incorporation of citric acid as a crosslinking agent is anticipated to enhance the barrier properties of the films. The combination of these additives holds promise for creating TPS films with improved performance, contributing to the development of sustainable and environmentally friendly materials in various industries. The results reveal that SiO2 improves the stiffness of the films at lower concentrations but causes brittleness at higher concentrations. In contrast, citric acid crosslinked films exhibit improved flexibility and density. Scanning electron microscopy demonstrates the morphological changes in the films, with SiO2 affecting surface roughness and aggregate formation. SiO2 reduces film thickness and transparency, while citric acid enhances water resistance and barrier properties. X-ray diffraction analysis shows a reduction in crystallinity due to the plasticization process. Fourier-transform infrared spectroscopy highlights chemical changes and antimicrobial activity is observed with citric acid against specific bacteria. The soil burial test reveals that citric acid crosslinked films exhibit slower degradation due to antimicrobial properties. The combination of SiO2 reinforcement and citric acid crosslinking enhances the overall performance of the films, promising sustainable and environmentally friendly materials for various applications. © 2024 The Royal Society of Chemistry.Item Microscopic and spectroscopic characterization of elastomer for microfluidics application(Optica Publishing Group (formerly OSA), 2020) Banik, S.; Pooja, N.; Chakraborty, I.; Mal, S.S.; Mahato, K.K.; Srisungsitthisunti, P.; Mazumder, N.We have developed elastomers using potato and corn starch with various concentration and characterised them using microscopic and spectroscopic techniques. © OSA 2020 © 2020 The Author(s)Item Microscopic and Thermal Characterization of Starch-Silicon Dioxide Elastomers(Optica Publishing Group (formerly OSA), 2022) Pooja, N.; Banik, S.; Chakraborty, I.; Mal, S.S.; Mahato, K.K.; Srisungsitthisunti, P.; Mazumder, N.Elastomers were synthesized from potato starch with silicon dioxide as a reinforcement filler. The films were further subjected to microscopic and thermal characterization. © 2022 The Author(s)Item Morphological and Thermal Characterization of Starch-Based Elastomers(Optica Publishing Group (formerly OSA), 2021) Pooja, N.; Banik, S.; Chakraborty, I.; Mal, S.S.; Mahato, K.K.; Srisungsitthisunti, P.; Mazumder, N.Novel elastomers are synthesized from corn and potato starch with potential application in the fabrication of biodegradable microfluidic devices. The developed elastomers were subjected to morphological and thermal characterization. © Optica Publishing Group 2021, © 2021 The Author (s)Item SYNTHESIS AND CHARACTERIZATION OF ACTIVATED CARBON-BASED STARCH BIOCOMPOSITE(Optical Society of America, 2023) Santhosh, N.P.; Pooja, N.; Mal, S.S.; Mazumder, N.Plastic overuse has resulted in severe environmental issues. Bioplastics, derived from biological sources like starch offer a potential solution to this problem. However, starch-based bioplastics show high susceptibility to moisture absorption, leading to poor mechanical strength. This study involves the use of activated carbon as reinforcement to improve the functional properties of starch bioplastics. © Optica Publishing Group 2023, © 2023 The Author(s)Item SYNTHESIS AND CHARACTERIZATION OF ACTIVATED CARBON-BASED STARCH BIOCOMPOSITE(Optical Society of America, 2023) Santhosh, N.P.; Pooja, N.; Mal, S.S.; Mazumder, N.Plastic overuse has resulted in severe environmental issues. Bioplastics, derived from biological sources like starch offer a potential solution to this problem. However, starch-based bioplastics show high susceptibility to moisture absorption, leading to poor mechanical strength. This study involves the use of activated carbon as reinforcement to improve the functional properties of starch bioplastics. © Optica Publishing Group 2023, © 2023 The Author(s)Item Synthesis and detailed characterization of sustainable starch-based bioplastic(John Wiley and Sons Inc, 2022) Chakraborty, I.; Pooja, N.; Banik, S.; Govindaraju, I.; Das, K.; Mal, S.S.; Zhuo, G.-Y.; Rather, M.A.; Mandal, M.; Neog, A.; Biswas, R.; Managuli, V.; Datta, A.; Mahato, K.K.; Mazumder, N.There is an urgent requirement of replacing the environmentally hazardous petroleum-based plastics with sustainable and efficient starch-based bioplastics. Development and detailed characterization of the biodegradable bioplastics from plant-based polysaccharides such as starch is essential to reduce plastic pollution in the environment. In this research, bioplastics were developed from an equivalent blend of starch from two different sources namely rice and potato (1:1, w/w), crosslinked with different concentrations of citric acid (CA). The effect of CA cross-linking of starch-based bioplastics was investigated on its physicochemical and functional properties. The X-ray diffraction (XRD) spectra revealed that the synthesized bioplastics were amorphous in nature with broad diffraction peaks. Further, the peak at 1716 cm−1 in Fourier transform infrared (FTIR) spectra indicated the formation of ester bonds in CA cross-linked bioplastics. Atomic force microscopy (AFM) revealed the surface roughness of the bioplastics decreased with increasing concentration of CA. Mechanical and thermal properties of bioplastics were characterized using universal testing machine, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA), respectively. © 2022 Wiley Periodicals LLC.