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https://idr.nitk.ac.in/jspui/handle/123456789/12850
Title: | Scale-up of naringinase production process based on the constant oxygen transfer rate for a novel strain of Bacillus methylotrophicus |
Authors: | Raval, K. Gehlot, K. Belur, P.D. |
Issue Date: | 2017 |
Citation: | Preparative Biochemistry and Biotechnology, 2017, Vol.47, 2, pp.192-198 |
Abstract: | Naringinase bioprocess based on Bacillus methylotrophicus was successfully scaled up based on constant oxygen transfer rate (OTR) as the scale-up criterion from 5-L bioreactor to 20-L bioreactor. OTR was measured in 5 and 20-L bioreactor under various operating conditions using dynamic method. The operating conditions, where complete dispersion was observed were identified. The highest OTR of 0.035 and 0.04 mMol/L/s was observed in 5 and 20-L bioreactor, respectively. Critical dissolved oxygen concentration of novel isolated strain B. methylotrophicus was found to be 20% of oxygen saturation in optimized medium. The B. methylotrophicus cells grown on sucrose had maximum oxygen uptake rate of 0.14 mMol/L/s in optimized growth medium. The cells produced the maximum naringinase activity of 751 and 778 U/L at 34 hr in 5 and 20-L bioreactors, respectively. The maximum specific growth rate of about 0.178/hr was observed at both the scales of operations. The maximum naringinase yield of 160 and 164 U/g biomass was observed in 5 and 20-L bioreactors, respectively. The growth and production profiles at both scales were similar indicating successful scale-up strategy for B. methylotrophicus culture. 2017 Taylor & Francis. |
URI: | http://idr.nitk.ac.in/jspui/handle/123456789/12850 |
Appears in Collections: | 1. Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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3.Scale-up of naringinase.pdf | 1.2 MB | Adobe PDF | ![]() View/Open |
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