![](/jspui/image/dspace_header.jpg)
Please use this identifier to cite or link to this item:
https://idr.nitk.ac.in/jspui/handle/123456789/12850
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Raval, K. | - |
dc.contributor.author | Gehlot, K. | - |
dc.contributor.author | Belur, P.D. | - |
dc.date.accessioned | 2020-03-31T08:42:16Z | - |
dc.date.available | 2020-03-31T08:42:16Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Preparative Biochemistry and Biotechnology, 2017, Vol.47, 2, pp.192-198 | en_US |
dc.identifier.uri | http://idr.nitk.ac.in/jspui/handle/123456789/12850 | - |
dc.description.abstract | Naringinase bioprocess based on Bacillus methylotrophicus was successfully scaled up based on constant oxygen transfer rate (OTR) as the scale-up criterion from 5-L bioreactor to 20-L bioreactor. OTR was measured in 5 and 20-L bioreactor under various operating conditions using dynamic method. The operating conditions, where complete dispersion was observed were identified. The highest OTR of 0.035 and 0.04 mMol/L/s was observed in 5 and 20-L bioreactor, respectively. Critical dissolved oxygen concentration of novel isolated strain B. methylotrophicus was found to be 20% of oxygen saturation in optimized medium. The B. methylotrophicus cells grown on sucrose had maximum oxygen uptake rate of 0.14 mMol/L/s in optimized growth medium. The cells produced the maximum naringinase activity of 751 and 778 U/L at 34 hr in 5 and 20-L bioreactors, respectively. The maximum specific growth rate of about 0.178/hr was observed at both the scales of operations. The maximum naringinase yield of 160 and 164 U/g biomass was observed in 5 and 20-L bioreactors, respectively. The growth and production profiles at both scales were similar indicating successful scale-up strategy for B. methylotrophicus culture. 2017 Taylor & Francis. | en_US |
dc.title | Scale-up of naringinase production process based on the constant oxygen transfer rate for a novel strain of Bacillus methylotrophicus | en_US |
dc.type | Article | en_US |
Appears in Collections: | 1. Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
3.Scale-up of naringinase.pdf | 1.2 MB | Adobe PDF | ![]() View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.