Faculty Publications

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Author: search.filters.author.Iyyaswami, R.Subject: search.filters.subject.Purification

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    Recovery and Partial Purification of Bovine ?-Lactalbumin from Whey Using PEG 1000 – Trisdoium Citrate Systems
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Sivakumar, K.; Iyyaswami, R.
    The extraction of ?-Lactalbumin (?-La) and ?-Lactoglobulin (?-Lg) from whey have been carried out in the Aqueous Two Phase System (ATPS) composed of PEG 1000 – tri sodium citrate. The suitable conditions for the simultaneous partitioning of ?-La into the top phase and ?-Lg in bottom phases was identified using the pure ?-La and ?-Lg. Maximum partition coefficient (k) of 16.67 was achieved for ?-La at the system condition of 28% (w/w) PEG 1000-14% (w/w) tri sodium citrate at pH 8 and 40°C, whereas in this condition k of ?-Lg is 0.27. 30% (w/w) whey loaded to this system yielded the maximum recovery of ?-La and ?-Lg. © , Copyright © Taylor & Francis Group, LLC.
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    Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Bolar, S.; Iyyaswami, R.; Belur, P.D.
    L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
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    Aqueous two phase based selective extraction of mannose/glucose specific lectin from Indian cultivar of Pisum sativum seed
    (Elsevier B.V., 2019) Rashmi, B.S.; Iyyaswami, R.
    Pisum sativum lectin (Psl) being a high-value protein has marked its application in the biomedical and therapeutic field. Aqueous two phase extraction (ATPE) was implemented as a selective partitioning technique for the partial purification of Psl from its seeds. PEG/citrate based biodegradable aqueous two phase system (ATPS) was screened and the factors such as the type and concentration of citrate salts, molar mass and concentration of polyethylene glycol (PEG), tie line length (TLL) and additive (NaCl) concentration, pH, crude load and volume ratio were studied for the selective partition of Psl. The Psl was successfully extracted to the top phase in the ATPS formed with 18% PEG 6000/16% sodium citrate at 41.01% TLL, 2% NaCl and pH of 7.5. A volume ratio of 0.76 and a crude load of 20% showed maximum activity yield of 122.12% with the purification factor of 16.26. The subunits of Psl namely ? and ? were identified with a molecular weight of 6 and 18 kDa respectively during the purity analysis using SDS PAGE and HPLC. © 2019 Elsevier B.V.
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    Biosurfactant Based Reverse Micellar Extraction of Lactoperoxidase from Whey: Exploitation of Rhamnolipid Characteristics for Back Extraction
    (Taylor and Francis Ltd., 2023) Karanth, S.; Iyyaswami, R.; Raj, N.T.
    Biosurfactant-based reverse micellar extraction of Lactoperoxidase (LP) was studied using Rhamnolipid (RL) as a biosurfactant. Different solvents were considered to select a suitable organic phase for forming reverse micelles (RM) to varying concentrations of RL for the extraction of LP from its synthetic aqueous solution. The effect of addition of nonionic surfactant as lipophilic linker, whey pH, and ionic strength of the whey was studied to improve the forward extraction of LP from acid whey. About 96.65% LP was extracted to the RM phase during forward extraction. Further, a new back extraction strategy was developed by harnessing the biosurfactant properties. The pH-specific protonation–deprotonation characteristic of the RL headgroups was exploited to overcome the back extraction of LP, which is the rate-limiting step. The back extraction in citrate buffer at pH 5 using 0.75 M KCl resulted in 85.71% active LP recovery with 8.4-fold purification. The effect of the extraction process on the antimicrobial activity of LP was further examined with S. aureus, and the multiplication of the organism was almost arrested even after 24 hr at 9°C. © 2023 Taylor & Francis Group, LLC.
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    Evaluation of Protein-polysaccharide complexes for microencapsulation of anthocyanins from Garcinia indica
    (Springer, 2025) Chandrakant Shanbhag, C.; N.g, K.; Iyyaswami, R.; D Belur, P.
    Microencapsulation of Anthocyanins (ACNs) extracted from dried rinds of Garcinia indica (GI) was performed using different polysaccharides (carboxy methyl cellulose, xanthan gum, inulin, chitosan, and gum arabic) in combination with two different proteins (whey protein isolate and sodium caseinate). The whey protein isolate (WPI)-xanthan gum (XG) complex was found to most efficiently encapsulate ACNs; Encapsulation Efficiency (EE) of 94.33% ±1.07 was achieved at 1% (w/v) of WPI, 1.50% (w/v) XG, 0.02 mg/mL ACNs, and pH 3. A comparative study on EE of ACNs from different sources, including standard ACN, GI crude ACNs, and Three-Liquid-Phase Extraction System (TLPES) purified ACNs, before (in the solution) and after freeze-drying (powder), indicated that the encapsulation is stable even after the freeze-drying of the WPI-ACNs-XG microencapsulates. Physicochemical characterization of freeze-dried ACNs microencapsulates done using antioxidant activity assay, particle size analysis, Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermo gravimetric analysis supported the higher compatibility of the WPI-XG complex to encapsulate ACNs. ACNs obtained from TLPES purification were the most suitable for encapsulation as compared to standard ACN and GI crude ACNs due to their higher antioxidant potential (71.10% ± 1.32) and smooth particle surface morphology that shield the ACNs from adverse surroundings. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.