Real-time interrogation of fiber optic biosensor using TiO2coated etched long-period grating
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Date
2020
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Publisher
American Institute of Physics Inc.
Abstract
In this work, a TiO2 coated etched long-period grating (e-LPG) fiber optic biosensor is developed for the detection of Escherichia coli (E. coli) bacteria in food items. Label-free Escherichia coli bacteria monitoring is done over the detection range of 0 cfu/ml-50 cfu/ml using an advanced spectral interrogation mechanism. The thin film deposition of 40 nm TiO2 over the e-LPG is confirmed by the microscopy method, such as scanning electron microscopy. In our proposed biosensor design, T4-bacteriophage is covalently immobilized over the TiO2 coated fiber surface. This biosensor system has reached sensitivity at 2.55 nm/RIU. Our experiments confirm the resolution and the limit of detection (3?/S) of 0.0039 RIU and 10.05 ppm, respectively. The proposed biosensor with enhanced sensitivity is suitable for monitoring harmful pathogens/infectious agents in various food products. © 2020 Author(s).
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Keywords
Deposition, Escherichia coli, Fiber optic sensors, Fiber optics, Food products, Oxide minerals, Scanning electron microscopy, Titanium dioxide, Bio-sensor systems, Enhanced sensitivity, Escherichia coli (E. coli), Escherichia coli bacteria, Fiber optic biosensor, Limit of detection, Long Period Gratings, Thin-film depositions, Biosensors, optic nerve fiber, titanium, titanium dioxide, devices, food control, genetic procedures, isolation and purification, limit of detection, refractometry, Biosensing Techniques, Food Microbiology, Limit of Detection, Optical Fibers, Refractometry, Titanium
Citation
Review of Scientific Instruments, 2020, 91, 12, pp. -