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Author: search.filters.author.Nainegali, B.S.Subject: search.filters.subject.Garcinia indica

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    Simultaneous extraction of four different bioactive compounds from Garcinia indica and their enrichment using Aqueous Two-Phase Systems
    (Institution of Chemical Engineers, 2019) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    Garcinia indica fruits contain several important bioactive compounds like anthocyanin, garcinol, isogarcinol and hydroxycitric acid. Simultaneous extraction of these bioactive compounds from the dried G. indica fruit by employing water, acidified water and an aqueous mixture of ethanol and propanol as solvents and subsequent enrichment using Aqueous Two-Phase Systems (ATPS) were studied. Aqueous 1-propanol (60% (v/v)) and aqueous ethanol (80% (v/v)) were found to be a superior solvent to extract anthocyanin, garcinol, isogarcinol and HCA compared to acidified water and water alone as solvent. The crude extract was further subjected to the PEG-salt and alcohol–salt based Aqueous Two-Phase Extraction (ATPE) for the simultaneous enrichment of garcinol, isogarcinol into top phase and anthocyanins and hydroxycitric acid towards the bottom phase. The ATPS containing ethanol and ammonium sulfate system with TLL of 38.60–43.28% was found suitable to enrich 86.33% of anthocyanins and 75.17% of HCA in salt-rich bottom phase and 96.39% of garcinol and 94.26% of isogarcinol in ethanol-rich top phase. © 2019 Institution of Chemical Engineers
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    Alcohol-based aqueous biphasic system applied to partition four different natural bioactive compounds from Garcinia indica Choisy
    (Bellwether Publishing, Ltd., 2021) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. The purity and recovery were further improved by employing the second stage ATPS. © 2020 Taylor & Francis Group, LLC.