Faculty Publications

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Author: search.filters.author.Iyyaswami, R.Subject: search.filters.subject.Response surface methodology

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    Synergistic extraction of ?-Lactalbumin and ?-Lactoglobulin from acid whey using aqueous biphasic system: Process evaluation and optimization
    (Elsevier, 2015) Kalaivani, S.; Iyyaswami, R.
    The present investigation focused on the simultaneous partitioning of ?-Lactalbumin (?-La) to the PEG rich phase and ?-Lactoglobulin (?-Lg) to the salt rich bottom phase from acid whey using polyethylene glycol 1000-trisodium citrate systems. The effect of Tie Line Length (TLL), pH, volume ratio and whey concentration on the proteins partitioning was evaluated. Further the Response Surface Methodology (RSM) was employed to analyze the synergistic and interactive effect of these variables (TLL, pH and whey loading) on the responses (recovery and purity of ?-La and ?-Lg). Optimum values of these variables were obtained for different goals through desirability based multi response optimization. A system of 54.12% (w/w) TLL with 30.6% (w/w) whey at pH 7.23 separates ?-La with 89% recovery and purity of 96% in the top phase, whereas that of ?-Lg in the bottom phase was 96% and 76% respectively. © 2015 Elsevier B.V.
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    Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Bolar, S.; Iyyaswami, R.; Belur, P.D.
    L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
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    Selective encapsulation of quercetin from dry onion peel crude extract in reassembled casein particles
    (Institution of Chemical Engineers, 2019) Ghatak, D.; Iyyaswami, R.
    Quercetin, a lipophilic dietary flavonoid, used as a therapeutic agent and a food component was encapsulated with casein particles to improve its water solubility as well as bioavailability in the food and pharmaceutical formulations. The nano-structured casein particles were reassembled with pure quercetin from the synthetic solution and the encapsulation yield was assessed by studying the effect of pH and the concentration of casein and additives like salts and Cetyl trimethylammonium bromide (CTAB). A maximum encapsulation yield of 97% was obtained for the reassembled casein particles formed with the addition of 0.5% (w/v) sodium caseinate, 0.1 M of calcium chloride, 0.5 M of di potassium hydrogen phosphate, 0.1 mM CTAB and 1 M of sodium citrate at a pH of 7. The identified process condition was further used to encapsulate the quercetin from the aqueous crude extract of dried onion peels. The microwave-assisted extraction was able to produce the crude extract with maximum quercetin content of 39.37 ?M per ml of the extract. Further the response surface methodology (RSM) was used to optimize the significant encapsulation variables for the maximum encapsulation yield of quercetin from aqueous crude extract. A maximum encapsulation yield of 96% was achieved at the pH 7.09 by using 2.1% (w/v) of sodium caseinate. © 2019 Institution of Chemical Engineers
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    Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization
    (Elsevier B.V., 2020) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS. © 2020 Elsevier B.V.