Faculty Publications

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Author: search.filters.author.Bolar, S.

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    Partitioning studies of glutaminase in polyethylene glycol and salt-based aqueous two-phase systems
    (2013) Bolar, S.; Belur, P.D.; Iyyaswami, I.
    The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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    Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Bolar, S.; Iyyaswami, R.; Belur, P.D.
    L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.