Faculty Publications

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Author: search.filters.author.Belur, P.D.Subject: search.filters.subject.Solvent extraction

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    Partitioning studies of glutaminase in polyethylene glycol and salt-based aqueous two-phase systems
    (2013) Bolar, S.; Belur, P.D.; Iyyaswami, I.
    The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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    Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Bolar, S.; Iyyaswami, R.; Belur, P.D.
    L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
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    Simultaneous extraction of four different bioactive compounds from Garcinia indica and their enrichment using Aqueous Two-Phase Systems
    (Institution of Chemical Engineers, 2019) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    Garcinia indica fruits contain several important bioactive compounds like anthocyanin, garcinol, isogarcinol and hydroxycitric acid. Simultaneous extraction of these bioactive compounds from the dried G. indica fruit by employing water, acidified water and an aqueous mixture of ethanol and propanol as solvents and subsequent enrichment using Aqueous Two-Phase Systems (ATPS) were studied. Aqueous 1-propanol (60% (v/v)) and aqueous ethanol (80% (v/v)) were found to be a superior solvent to extract anthocyanin, garcinol, isogarcinol and HCA compared to acidified water and water alone as solvent. The crude extract was further subjected to the PEG-salt and alcohol–salt based Aqueous Two-Phase Extraction (ATPE) for the simultaneous enrichment of garcinol, isogarcinol into top phase and anthocyanins and hydroxycitric acid towards the bottom phase. The ATPS containing ethanol and ammonium sulfate system with TLL of 38.60–43.28% was found suitable to enrich 86.33% of anthocyanins and 75.17% of HCA in salt-rich bottom phase and 96.39% of garcinol and 94.26% of isogarcinol in ethanol-rich top phase. © 2019 Institution of Chemical Engineers
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    Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization
    (Elsevier B.V., 2020) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS. © 2020 Elsevier B.V.