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Author: search.filters.author.Belur, P.D.Subject: search.filters.subject.Aqueous two phase system

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    Development and Evaluation of PEG-Lithium Citrate Salt Based Aqueous Two Phase System and Its Application in Partitioning of Proteins from Fish Industry Effluent
    (2012) Iyyaswami, I.; Belur, P.D.; Girish, B.; Nagaraj, V.H.
    A aqueous two phase system (ATPS) comprising of PEG (Average mol. Wt: 4000, 6000, 8000) - lithium citrate salt-water systems were studied. The basic studies like binodal curve data generation and equilibrium studies were carried out. Furthermore, the binodal model and Othmer-Tobias and Bancroft models for phase equilibria were used for reproducing the experimental binodal data and phase equilibrium composition data, respectively. Good agreement was obtained with the experimental binodal data and tie line data with the models. The effective excluded volume values were obtained from the binodal model for the present ATPS. The tie line length was determined through the phase equilibrium composition data. This system was used to partition crude proteins of the fish industry effluent. The effects of PEG and salt weight fraction in terms of tie line length and effective excluded volume on partitioning coefficient of crude protein were studied in detail. From the results it was observed that, the crude proteins present in the fish effluent were partitioned in the PEG rich phase and the maximum partition coefficient of 7.82 was obtained. The results are discussed in the context of practical potential of this citrate based ATPS in separating crude proteins from fish industry effluent. © 2012 Copyright Taylor and Francis Group, LLC.
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    Partitioning studies of glutaminase in polyethylene glycol and salt-based aqueous two-phase systems
    (2013) Bolar, S.; Belur, P.D.; Iyyaswami, I.
    The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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    Purification of Glutaminase from Zygosaccharomyces rouxii in Polyethylene Glycol– Sodium Sulphate Aqueous Two-Phase System
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2015) Bolar, S.; Iyyaswami, R.; Belur, P.D.
    L-glutaminase (EC 3.5.1.2) produced from Zygosaccharomyces rouxii NRRL-Y 2547 was partitioned in an aqueous two phase system comprising PEG 2000 and sodium sulphate. The effects of tie line length (TLL), pH, broth loading (BL), volume ratio, and neutral salt concentration on enzyme partitioning and purification were investigated. The optimal condition for the partitioning of glutaminase was obtained through response surface methodology and obtained the partition coefficient and yield of 12.99 and 95.12%, respectively. The purification factor of 5.59 and selectivity of 6.52 were achieved at the optimal condition. © © Taylor & Francis Group, LLC.
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    Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization
    (Elsevier B.V., 2020) Nainegali, B.S.; Iyyaswami, R.; Belur, P.D.
    The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS. © 2020 Elsevier B.V.