Dubey, A.P.Thalla, A.K.2026-02-032025Journal of Hazardous Materials, 2025, 487, , pp. -3043894https://doi.org/10.1016/j.jhazmat.2025.137139https://idr.nitk.ac.in/handle/123456789/20328Plastic pollution, especially microplastics (MPs), is a severe environmental threat. Due to the significant environmental issues posed by plastics, it is critical to use an effective and sustainable degradation technique. The study aimed to isolate and identify Indigenous bacterial strains from landfill leachate (LL) to evaluate its potential for degrading Polypropylene microplastics (PPMPs). The investigation identified two bacterial strains, Pseudomonas aeruginosa, and novel Staphylococcus haemolyticus, through 16S rRNA analysis, capable of decomposing PPMPs. Following a 30-day treatment period, it was noted that Staphylococcus haemolyticus reduced the dry weight of PPMPs by 25.46 % ± 1.35 %, whereas Pseudomonas aeruginosa strain reduced it by 7.01 % ± 0.85 %. Multiple tests, including weight loss, pH, optical density, total biomass yield, and BATH test of the medium, validated the growth of bacterial strains. The biochemical characteristics of the isolated strains were assessed through Biochemical tests. The study also investigated the surface, chemical, and structural changes in treated PPMPs using Scanning electron microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDS), X-ray diffractometer (XRD), and Ion Chromatography (IC) tests. The Fourier Transform Infrared Spectroscopy (FTIR) study also showed the creation of alcohol, methyl, as well as carbonyl groups due to hydrolysis and oxidation by both bacterial strains. This study implies that the Staphylococcus haemolyticus and Pseudomonas aeruginosa bacterial strains are secure and efficient for PPMP bioremediation. © 2025 Elsevier B.V.BioremediationFourier transform infrared spectroscopyIon chromatographyLeachate treatmentMicroplasticPolypropylenesX ray diffraction analysisX ray spectrometers16S rRNABacterial strainsDumpsiteFourier transform infraredIsolationLandfill leachatesMicroplasticsPseudomonas aeruginosaStaphylococcus haemolyticusTransform infrared spectroscopyEnergy dispersive spectroscopyalcoholcarbonyl derivativecatalasecitric acidDNA fragmentdodecyl sulfate sodiummicroplasticoxidoreductasepolypropyleneRNA 16Sbiomassdegradationdetection methodoxidationplasticRNAX-ray spectroscopyArticlebacterial growthbacterial strainbacterium colonybacterium isolationbiodegradationbioprospectingbioremediationbody weight losscarbon sourcecell adhesioncell surfacecontrolled studycytolysisdry weightenergy dispersive X ray spectroscopyenzymatic hydrolysisenzyme activityfirst order rate constanthydrolysishydrophobicityion chromatographylandfill leachatemaximum likelihood methodmicroplastic pollutionnative speciesnonhumanoptical densitypHreproducibilityscanning electron microscopystatic electricityX ray diffractionchemistrygeneticsmetabolismwaste disposal facilitywater pollutantBiodegradation, EnvironmentalRNA, Ribosomal, 16SWaste Disposal FacilitiesWater Pollutants, Chemical