Borah, A.J.Dikshit, P.K.Doloi, M.Moholkar, V.S.Poddar, M.K.2026-02-042023Biomass Conversion and Biorefinery, 2023, 13, 12, pp. 11121-1113021906815https://doi.org/10.1007/s13399-021-01960-6https://idr.nitk.ac.in/handle/123456789/21819This study has put forth the concept of “control through utilization” for invasive weeds, which cause severe damage to ecosystem and biodiversity. The study comprised of extraction of lignin and pretreatment of spent biomass of four invasive weeds. Lignin was extracted from native biomass using 1,4–dioxane as solvent. Lignin yield of all biomass varied in the range 8.61 − 11.14% w/w with the solvent recovery of 75.56–78.88% v/v. Extracted lignin was characterized using FTIR, TGA, and GPC. Lignin from Mikania micrantha had the highest thermal stability (T<inf>50%</inf> = 490 °C) and molecular weight (M<inf>w</inf> = 5537 g/mol). The spent biomass was subjected to dilute acid pretreatment for synthesis of total reducing sugars (TRS). TRS yields from different entities were as follows: non-extracted biomass = 94.18 − 221.81 mg/g, warm water wash = 8.74 − 15.66 mg/g, and raw biomass = 115.9 − 391.27 mg/g raw biomass. Biomass of Saccharum spontaneum gave the highest TRS yield. Graphical abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.BiodiversityBiomassLigninMolecular weightPolyolsSolventsThermodynamic stabilityDilute acid pretreatmentFTIR-TGAHigh thermal stabilityInvasive weedPre-treatmentsSolvent recoverySugar yieldTotal reducing sugarsWarm waterWater washSolvent extractionExtractionMolecular Weight