Narayani, M.Shetty K, K.V.2026-02-052012Journal of Microbiology and Biotechnology, 2012, 22, 5, pp. 690-69810177825https://doi.org/10.4014/jmb.1110.10073https://idr.nitk.ac.in/handle/123456789/27018Cr-B2, a Gram-uegadve hexavalent chromium [Cr(VI)] reducing bacteria, was isolated from the aerator water of an activated sludge process in the wastewater treatment facility of a dye and pigment based chemical industry. Cr-B2 exhibited a resistance for 1,100mg/l Cr(VI) and, similarly, resistance against other heavy metal ions such as Ni2+ (800 mg/l), Cu2+ (600 mg/l), Pb2+ (1,100 mg/l), Cd2+ (350 mg/l), Zn2+ (700 mg/l), and Fe3+ (1,000 mg/l), and against selected antibiotics. Cr-B2 was observed to efficiently reduce 200mg/l Cr(VI) completely in both nutrient and LB media, and could convert Cr(VI) to Cr(III) aerobically. Cr(VI) reduction kinetics followed allosteric enzyme kinetics. The K<inf>m</inf> values were found to be 43.11 mg/l for nutrient media and 38.05 mg/l for LB media. V<inf>max</inf> values of 13.17 mg/l/h and 12.53 mg/l/h were obtained for nutrient media and LB media, respectively, and the cooperativity coefficients (n) were found to be 8.47 and 3.49, respectively, indicating positive cooperativity in both cases. SEM analysis showed the formation of wrinkles and depressions in the cells when exposed to 800 mg/l Cr(VI) concentration. The organism was seen to exhibit pleomorphic behavior. Cr-B2 was identified on the basis of morphological, biochemical, and partial 16S rRNA gene sequencing chracterizations and found to be Acinetobacter sp. © The Korean Society for Microbiology and Biotechnology.cadmiumchloramphenicolchromiumcupric ionferric iongentamicinhexavalent chromiumkanamycinleadnalidixic acidnickelnovobiocinRNA 16Sstreptomycinunclassified drugzinc ionAcinetobacterAcinetobacter Cr B2antibiotic resistanceantibiotic sensitivityarticlebacterial genebacterial growthbacterial strainbacterium culturebacterium identificationbacterium isolationbioremediationbiotransformationcell structurecontrolled studyculture mediumenzyme kineticsgene sequencemetal tolerancemicrobial morphologyminimum inhibitory concentrationnonhumannucleotide sequencereduction kineticsscanning electron microscopysequence homologyBiodegradation, EnvironmentalChromiumKineticsMolecular Sequence DataOxidation-ReductionSewageWater Pollutants, ChemicalAcinetobacter sp.