Santosh Kumar, B.Y.Kumar, G.C.Shahapurkar, K.Tirth, V.Algahtani, A.Al-Mughanam, T.Alghtani, A.H.Murthy, H.C.2026-02-042023Journal of the Mechanical Behavior of Biomedical Materials, 2023, 144, , pp. -17516161https://doi.org/10.1016/j.jmbbm.2023.105963https://idr.nitk.ac.in/handle/123456789/21797The present work is focused on the nano-Hydroxyapatite (nHAp) synthesis with two different Indian breed Aseel and Kadaknath eggshells. The alloplast implants were developed through the foam replica method with polyurethane 45-PPI as a porous template. The synthesized nHAp was characterized by Field Emission Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The FE-SEM images of the nHAp showed the one dimensional clustered nanoparticles and the X-ray diffraction spectrum confirms that the major phase was hydroxyapatite with a small trace of β-tricalcium phosphate. The maximum compression strength of the sample was 5.49 ± 0.12 MPa which is in the range of the compression strength of human trabecular bone. The thermal and degradability studies results confirmed that these are highly stable and provides necessary a resorption needed for new bone tissue formation. Besides, the antimicrobial activity against tested human microbiome are satisfactory and the cell viability towards MG 63 human osteoblast-like cells provides a potential pathway for developing the nHAp implants for bone tissue engineering. © 2023 Elsevier LtdBoneCell engineeringEnamelsField emission microscopesFourier transform infrared spectroscopyNanocompositesScanning electron microscopyTissue engineeringX ray diffractionAlloplastCompression strengthEgg shellsField emission scanning electron microscopyHydroxyapatite synthesisNano-hydroxyapatitePorous templatesReplica methodSynthetic boneTrabecular bone tissueHydroxyapatitecalcium phosphatehydroxyapatitenanohydroxyapatitepolyurethanN-hydroxy-2-aminopyreneantimicrobial activityarthroscopyArticlebiodegradabilitybone tissueCandida albicanscell viabilitycompressioncontrolled studyegg shellEscherichia colifield emission scanning electron microscopyfoamhumanhuman cellMG-63 cell linemicrobiomenonhumanossificationosteoblastporosityStaphylococcus aureusstrengthsynthesistrabecular boneanimalbonechemistrydentistryorthopedicsprocedurestissue engineeringAnimalsArthroscopyBone and BonesDentistryDurapatiteEgg ShellHumansOrthopedicsSpectroscopy, Fourier Transform InfraredTissue EngineeringTissue ScaffoldsX-Ray Diffraction