Rashmi, B.S.Iyyaswami, R.2026-02-052019Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2019, 1114-1115, , pp. 13-2315700232https://doi.org/10.1016/j.jchromb.2019.03.019https://idr.nitk.ac.in/handle/123456789/24582Pisum sativum lectin (Psl) being a high-value protein has marked its application in the biomedical and therapeutic field. Aqueous two phase extraction (ATPE) was implemented as a selective partitioning technique for the partial purification of Psl from its seeds. PEG/citrate based biodegradable aqueous two phase system (ATPS) was screened and the factors such as the type and concentration of citrate salts, molar mass and concentration of polyethylene glycol (PEG), tie line length (TLL) and additive (NaCl) concentration, pH, crude load and volume ratio were studied for the selective partition of Psl. The Psl was successfully extracted to the top phase in the ATPS formed with 18% PEG 6000/16% sodium citrate at 41.01% TLL, 2% NaCl and pH of 7.5. A volume ratio of 0.76 and a crude load of 20% showed maximum activity yield of 122.12% with the purification factor of 16.26. The subunits of Psl namely ? and ? were identified with a molecular weight of 6 and 18 kDa respectively during the purity analysis using SDS PAGE and HPLC. © 2019 Elsevier B.V.Antigen-antibody reactionsMolar ratioPlants (botany)ProteinsPurificationSodium chlorideSolvent extractionAqueous two phase systemAqueous two-phase extractionPartial purificationPartitioning coefficientsPartitioning techniquesPurification factorsSelective extractionTie lineMolar concentrationadenosine triphosphatecitrate potassiumcitrate sodiumglucoselectinmacrogolmacrogol 6000mannosePisum sativum extractplant extractunclassified drugmannose binding lectinplant lectinArticlecultivarhemagglutinationhigh performance liquid chromatographyhydrophobicitymolecular weightnonhumanpeapolyacrylamide gel electrophoresispriority journalchemistryisolation and purificationliquid liquid extractionpHplant seedproceduresChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelHydrogen-Ion ConcentrationLiquid-Liquid ExtractionMannose-Binding LectinsPeasPlant LectinsPolyethylene GlycolsSeedsSodium Citrate