Rao, L.N.Shetty, A.Isloor, A.M.Nayak, S.S.Kumar, M.Shetty K, J.Venkatesh, M.2026-02-032025Journal of Oral Biology and Craniofacial Research, 2025, 15, 4, pp. 737-74322124268https://doi.org/10.1016/j.jobcr.2025.04.001https://idr.nitk.ac.in/handle/123456789/20212Background: Root perforation represents a significant complication in endodontics, compromising the structural integrity of the tooth. Effective repair are critical to mitigating the associated risks and preserving long-term dental function. Despite extensive research, the ideal repair material that fulfills all requisite biological and mechanical properties remains elusive. Methodology: This study introduces a novel polydopamine based repair material designed to address the limitations of existing materials. The material was characterized using field emission scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, transmission electron microscopy, zeta potential, and particle size analysis. The material's initial and final setting times were evaluated using a Gilmore needle apparatus. Biocompatibility was assessed via the MTT assay, while bioactivity was quantified through calcium ion release analysis. Comparative evaluations were conducted against ProRoot MTA, a commercially available standard in root repair. Results: The findings revealed no statistically significant differences in cytotoxicity (P > 0.05) between the experimental material and ProRoot MTA, affirming the biocompatibility of both. However, the experimental material demonstrated a significantly faster set (P < 0.001) than ProRoot MTA, a critical advantage in clinical applications. the inclusion of calcium chloride and bioactive glass enhanced calcium ion release, with the experimental material showing significantly higher levels at 7 days. Conclusion: The polydopamine-bioactive glass composite exhibited favorable biological activity, superior setting dynamics, and enhanced bioactivity, positioning it as a promising candidate for perforation repair. These results lay the groundwork for further research and potential clinical translation, offering a viable solution to a longstanding challenge in endodontics. © 2025 The AuthorsBioactive glassBiocompatibilityEndodontic materialsPolydopamineRoot perforation repair