Susanna, D.Mohan Balakrishnan, R.M.JagadeeshBabu, J.2026-02-042023Journal of Drug Delivery Science and Technology, 2023, 87, , pp. -17732247https://doi.org/10.1016/j.jddst.2023.104740https://idr.nitk.ac.in/handle/123456789/21747Nanotechnology and biogenic synthesis of metal nanoparticles using plants have attracted immense attention recently owing to their beneficial applications. Ultrasonication technique was employed for the rapid one-step phytochemical synthesis of AuNPs from the extracts of Nothapodytes foetida leaves. The N. foetida AuNPs exhibited the characteristic UV absorption peak at 524 nm. Several analytical techniques, including FESEM, TEM, XRD, FTIR, TGA, ICP and zeta potential analysis, were performed for their effective characterization. These analyses revealed highly stable (– 60.7 mV) crystalline AuNPs, majorly spherical, ranging between 5 and 30 nm. Furthermore, CCD-RSM was incorporated to determine the influence of the temperature, N. foetida leaf extract and HAuCl<inf>4</inf> concentration on the synthesis of AuNPs. LC-MS revealed the phytocompounds that contributed to AuNP synthesis which was subsequently confirmed by TPC and TFC reduction. Potent radical scavenging ability accompanied by the antibacterial activity of the AuNPs was also evaluated. Importantly, AuNPs also reduced the cell viability of MG63 and A549 cell lines, exhibiting significant anticancer activity along with appreciable wound-healing properties. These findings suggest that these AuNPs possess tremendous biological potentialities and can be bio-prospected further for various multidisciplinary applications. © 2023 Elsevier B.V.2,6 dibromophenol3 phosphoglyceric acid9 methoxycamptothecinapigeninepicatechingallic acidglucose 1 phosphategold chloridegold nanoparticlekaempferideluteolinNothapodytes foetida extractpara coumaric acidphytochemicalplant extractstigmasterolstreptomycintriacylglycerolunclassified drugA-549 cell lineABTS radical scavenging assayantibacterial activityantineoplastic activityantioxidant activityArticlebiosynthesiscell viabilityconcentration (parameter)controlled studydispersityDPPH radical scavenging assayenergy dispersive X ray spectroscopyfield emission scanning electron microscopyFourier transform infrared spectroscopygreen chemistryhigh resolution transmission electron microscopyhydrogen peroxide scavenging assayIC50in vitro studyliquid chromatography-mass spectrometrymedicinal plantMG-63 cell lineminimum inhibitory concentrationnanobiotechnologyNCTC clone 929 cell linenonhumanNothapodytes foetidaparticle sizephoton correlation spectroscopyphysical chemistryplant leafprecursorproapoptotic activityreaction duration (chemistry)reaction optimizationreaction temperatureresponse surface methodsurface chargesurface plasmon resonancethermogravimetryultrasoundultraviolet visible spectroscopywound healingX ray diffractionzeta potential