Bolar, S.Belur, P.D.Iyyaswami, I.2026-02-052013Chemical Engineering and Technology, 2013, 36, 8, pp. 1378-13869307516https://doi.org/10.1002/ceat.201200677https://idr.nitk.ac.in/handle/123456789/26746The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Aqueous two phase systemBancroft equationGlutaminasePartitioningPhase componentPolyethylene glycol (PEG)Salt typesTie lineSolvent extractionPolyethylene glycols