Singh, B.Jana, S.K.Ghosh, N.Das, S.K.Joshi, M.Bhattacharyya, P.Chaudhury, K.2026-02-052017Journal of Pharmaceutical and Biomedical Analysis, 2017, 132, , pp. 103-1087317085https://doi.org/10.1016/j.jpba.2016.09.034https://idr.nitk.ac.in/handle/123456789/25707Serum metabolic profiling can identify the metabolites responsible for discrimination between doxycycline treated and untreated chronic obstructive pulmonary disease (COPD) and explain the possible effect of doxycycline in improving the disease conditions. 1H nuclear magnetic resonance (NMR)-based metabolomics was used to obtain serum metabolic profiles of 60 add-on doxycycline treated COPD patients and 40 patients receiving standard therapy. The acquired data were analyzed using multivariate principal component analysis (PCA), partial least-squares-discriminant analysis (PLS-DA), and orthogonal projection to latent structure with discriminant analysis (OPLS-DA). A clear metabolic differentiation was apparent between the pre and post doxycycline treated group. The distinguishing metabolites lactate and fatty acids were significantly down-regulated and formate, citrate, imidazole and L-arginine upregulated. Lactate and folate are further validated biochemically. Metabolic changes, such as decreased lactate level, inhibited arginase activity and lowered fatty acid level observed in COPD patients in response to add-on doxycycline treatment, reflect the anti-inflammatory action of the drug. Doxycycline as a possible therapeutic option for COPD seems promising. © 2016 Elsevier B.V.arginaseargininebeta 2 adrenergic receptor stimulating agentcitric acidcorticosteroiddoxycycline hyclatefatty acidformic acidimidazolelactic acidmuscarinic receptor blocking agentplacebodoxycyclinefolic acidlactic acid derivativeadd on therapyadultagedantiinflammatory activityArticlechronic obstructive lung diseaseclinical articlecontrolled studydiarrheadiscriminant analysisdrug effectdrug tolerabilityenzyme activityfollow upforced expiratory volumeforced vital capacityhumanlung functionmalemetabolitemetabolomicsmultivariate analysisnauseapartial least squares regressionprincipal component analysispriority journalprotein expressionproton nuclear magnetic resonancerandomized controlled trialstatistical analysistreatment outcomebloodchemistryleast square analysislungmass spectrometrymetabolismmetabolomemiddle agednuclear magnetic resonance spectroscopyproceduresPulmonary Disease, Chronic ObstructiveAdultAgedDiscriminant AnalysisDoxycyclineFatty AcidsFolic AcidHumansLactatesLeast-Squares AnalysisLungMagnetic Resonance SpectroscopyMaleMass SpectrometryMetabolomeMetabolomicsMiddle AgedMultivariate AnalysisPrincipal Component AnalysisTreatment Outcome