Nagaraja, V.H.Iyyaswami, R.2026-02-052015Journal of Food Science and Technology, 2015, 52, 6, pp. 3539-3548221155https://doi.org/10.1007/s13197-014-1425-4https://idr.nitk.ac.in/handle/123456789/26270A suitable Aqueous Two Phase System (ATPS) has been identified for the partitioning of crude fish proteins from fish processing industrial effluent. A detailed study has been performed to analyze the influence of various parameters on protein partitioning such as molecular weight of PEG, effect of different salts (MgSO4<inf>,</inf> K<inf>2</inf>HPO<inf>4,</inf> Na<inf>3</inf>C<inf>6</inf>H<inf>5</inf>O<inf>7,</inf> Na<inf>2</inf>SO<inf>4,</inf> (NH<inf>4</inf>) <inf>2</inf>PO<inf>4,</inf> K<inf>3</inf>C<inf>6</inf>H<inf>5</inf>O<inf>7</inf>) and their concentrations, pH, temperature, Tie Line Length (TLL), effluent loading and volume ratio. PEG 2000 - sodium sulphate ATPS was found to be a most favourable system among the selected ATPS for higher partition coefficient of proteins. The binodal curve and equilibrium characteristics of PEG 2000 - sodium sulphate were established and fitted to empirical equations. The equilibrium compositions (tie line) were correlated using Othmer–Tobias and Bancroft equations. © 2014, Association of Food Scientists & Technologists (India).EffluentsFishPhase diagramsSewageSolvent extractionAqueous two phase systemBancroft equationEmpirical equationsEquilibrium compositionsIndustrial effluentPartition coefficientPartitioning coefficientsProtein partitioningProteins