Production of oxalate oxidase from endophytic Ochrobactrum intermedium CL6

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dc.contributor.authorKumar, K.
dc.contributor.authorBelur, P.D.
dc.date.accessioned2026-02-05T09:30:50Z
dc.date.issued2018
dc.description.abstractFour oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect of nutritional and non-nutritional parameters showed that oxalate oxidase production is inducible, requires Manganese ions in the medium, and very low fill-up volume is beneficial. Shake flask fermentation carried out with medium comprising Sucrose, Ammonium chloride, Sodium oxalate along with basal salts gave 0.5 UmL-1 oxalate oxidase activity and 0.454 Umg-1specific activity after 65h of fermentation. © 2018 The Author(s).
dc.identifier.citationJournal of Pure and Applied Microbiology, 2018, 12, 4, pp. 2327-2336
dc.identifier.issn9737510
dc.identifier.urihttps://doi.org/10.22207/JPAM.12.4.75
dc.identifier.urihttps://idr.nitk.ac.in/handle/123456789/24890
dc.publisherJournal of Pure and Applied Microbiology micro_drkhan@yahoo.com 54, Near Post Office, Thana Street, Shahjahanabad Bhopal 462 001
dc.subjectammonium chloride
dc.subjectcalcium oxalate
dc.subjectcarbon
dc.subjectcasein hydrolysate
dc.subjectenzyme
dc.subjecthydrogen peroxide
dc.subjectinorganic salt
dc.subjectoxalate oxidase
dc.subjectsucrose
dc.subjectunclassified drug
dc.subjectArticle
dc.subjectbacterial growth
dc.subjectbiomass
dc.subjectcarbon source
dc.subjectColocasia esculenta
dc.subjectDNA sequence
dc.subjectenzyme activity
dc.subjectenzyme synthesis
dc.subjectfermentation
dc.subjectnonhuman
dc.subjectOchrobactrum
dc.subjectOchrobactrum intermedium
dc.subjectoxidation
dc.titleProduction of oxalate oxidase from endophytic Ochrobactrum intermedium CL6

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