Coconut coir pith, available in abundance especially in tropical countries, was studied as a substrate for the production of cellulase[1,4(1,3;1,4)???D?glucan 4?glucanohydrolase, EC 3.2.1.4] and ??D?glucosidase(??D?glucoside glucohydrolase, EC 3.2.1.21) in solid state fermentation. The effects of fermentation time, nutrient level, substrate particle size and inoculum size have been examined for optimal production of these enzymes by the fungal strain Aspergillus niger NCIM 1005. The highest filter paper activity (FPA) of 4.11 IU g?1, carboxyl methyl cellulose (CMCase) activity of 15·55 IU g?1 and cellobiase activity of 9·31 IU g?1 were obtained after 7 to 8 days of fermentation. Reese and Mandel's mineral solution in the substrate to mineral solution ratio of 1:10 (w/v) supported high cellulase and cellobiase activities. An inoculum size of 20–50% (v/v) based on the volume of mineral medium and substrate average particle size of 375 ?m were optimum for enzyme production. Copyright © 1994 SCI

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dc.contributor.authorMuniswaran, P.K.A.
dc.contributor.authorSelvakumar, P.
dc.contributor.authorCharyulu, N.C.L.N.
dc.date.accessioned2026-02-05T11:00:36Z
dc.date.issuedProduction of cellulases from coconut coir pith in solid state fermentation
dc.description.abstract1994
dc.identifier.citationJournal of Chemical Technology and Biotechnology, 1994, 60, 2, pp. 147-151
dc.identifier.issn2682575
dc.identifier.urihttps://doi.org/10.1002/jctb.280600206
dc.identifier.urihttps://idr.nitk.ac.in/handle/123456789/28080
dc.subjectAgricultural wastes
dc.subjectCellulose
dc.subjectEnzymes
dc.subjectFermentation
dc.subjectFungi
dc.subjectParticle size analysis
dc.subjectSubstrates
dc.subjectAspergillus niger
dc.subjectCarboxyl methyl cellulose
dc.subjectCellobiase
dc.subjectCellulase
dc.subjectCoconut coir pith
dc.subjectInoculum size
dc.subjectNutrient level
dc.subjectSolid state fermentation
dc.subjectCellulose derivatives
dc.subjectbeta glucosidase
dc.subjectcellulase
dc.subjectglucosidase
dc.subjectarticle
dc.subjectaspergillus niger
dc.subjectcoconut
dc.subjectenzyme activity
dc.subjectenzyme synthesis
dc.subjectfermentation
dc.subjectparticle size
dc.subjecttropics
dc.titleCoconut coir pith, available in abundance especially in tropical countries, was studied as a substrate for the production of cellulase[1,4(1,3;1,4)???D?glucan 4?glucanohydrolase, EC 3.2.1.4] and ??D?glucosidase(??D?glucoside glucohydrolase, EC 3.2.1.21) in solid state fermentation. The effects of fermentation time, nutrient level, substrate particle size and inoculum size have been examined for optimal production of these enzymes by the fungal strain Aspergillus niger NCIM 1005. The highest filter paper activity (FPA) of 4.11 IU g?1, carboxyl methyl cellulose (CMCase) activity of 15·55 IU g?1 and cellobiase activity of 9·31 IU g?1 were obtained after 7 to 8 days of fermentation. Reese and Mandel's mineral solution in the substrate to mineral solution ratio of 1:10 (w/v) supported high cellulase and cellobiase activities. An inoculum size of 20–50% (v/v) based on the volume of mineral medium and substrate average particle size of 375 ?m were optimum for enzyme production. Copyright © 1994 SCI

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