Rhodamine-based Cu2+-selective fluorosensor: Synthesis, mechanism, and application in living cells

Abstract

A rhodamine B-based fluorescence probe (1) for the sensitive and selective detection of Cu2+ ion has been designed and synthesized using pyridine moiety. The optical properties of this compound have been investigated in acetonitrile-water binary solution (7:3 v/v). Compound 1 is found to be an excellent sensor for a biologically/physiologically very important transition metal ion (Cu2+) using only the two very different modes of measurements (absorption and emission); one case displayed intensity enhancement whereas in other case showed intensity depletion (quenching). A mechanistic investigation has been performed to explore the static nature of quenching process. The sensor has been found to be very effective in sensing Cu 2+ ion inside living cells also. © 2013 Springer Science+Business Media New York.