Partitioning studies of ?-lactalbumin in environmental friendly poly (ethylene glycol) - Citrate salt aqueous two phase systems

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dc.contributor.authorKalaivani, S.
dc.contributor.authorIyyaswami, I.
dc.date.accessioned2026-02-05T09:34:41Z
dc.date.issued2013
dc.description.abstractAqueous two-phase systems (ATPS) formed by polymer and salt have been utilized to enrich the desired biomolecule into one of the phase with higher yield and purity. The eco-friendly, biodegradable poly ethylene glycol (PEG) and different citrate salts were chosen as ATPS phase components to investigate the partitioning behavior of ?-lactalbumin (?-La). System factors and process parameters such as type and concentration of salt, molecular weight and concentration of PEG, pH, temperature and the effect of additives were studied and the results are discussed in detail. PEG 1000-tri-potassium citrate system yields high partition coefficient of 20 with a better yield of 98 % in the top phase. The addition of NaCl as an additive and acidic pH lowers the yield of ?-La in the top phase. Influence of phase volume ratio (V <inf>r</inf>) on partitioning was studied and found that the partition coefficient remains almost constant along the tie line. High yield was achieved at a V <inf>r</inf> of 3.5 at the tie line length of 50.63 (%, w/w). © 2013 Springer-Verlag Berlin Heidelberg.
dc.identifier.citationBioprocess and Biosystems Engineering, 2013, 36, 10, pp. 1475-1483
dc.identifier.issn16157591
dc.identifier.urihttps://doi.org/10.1007/s00449-013-0910-x
dc.identifier.urihttps://idr.nitk.ac.in/handle/123456789/26728
dc.subjectAqueous two phase system
dc.subjectATPE
dc.subjectEnvironmental-friendly
dc.subjectPartition coefficient
dc.subjectPhase component
dc.subjectPhase volume ratio
dc.subjectProcess parameters
dc.subjectTri-potassium citrate
dc.subjectAdditives
dc.subjectEthylene glycol
dc.subjectSolvent extraction
dc.subjectPolyethylene glycols
dc.subjectalpha lactalbumin
dc.subjectcitrate potassium
dc.subjectmacrogol
dc.subjectsodium chloride
dc.subjectaqueous solution
dc.subjectarticle
dc.subjectbiodegradability
dc.subjectconcentration (parameters)
dc.subjectgreen chemistry
dc.subjecthydrophobicity
dc.subjectmolecular interaction
dc.subjectmolecular weight
dc.subjectpartition coefficient
dc.subjectpH measurement
dc.subjectphase partitioning
dc.subjectpriority journal
dc.subjectprotein analysis
dc.subjectsolubility
dc.subjecttemperature sensitivity
dc.subjectCitrates
dc.subjectHydrogen-Ion Concentration
dc.subjectLactalbumin
dc.subjectMolecular Weight
dc.subjectPolyethylene Glycols
dc.subjectSalts
dc.subjectTemperature
dc.subjectWater
dc.titlePartitioning studies of ?-lactalbumin in environmental friendly poly (ethylene glycol) - Citrate salt aqueous two phase systems

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