Partitioning studies of glutaminase in polyethylene glycol and salt-based aqueous two-phase systems
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Date
2013
Authors
Bolar, S.
Belur, P.D.
Regupathi, I.
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Abstract
The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated. 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Chemical Engineering and Technology, 2013, Vol.36, 8, pp.1378-1386