Total phenolics from an endophytic fungus Penicillium sp isolated from Nothapodytes foetida and its optimization

Abstract

Microwave Assisted Extraction (MAE) of total phenolic content (TPC) from an endophytic fungus Penicillium sp. was carried out and the conditions for extraction was optimized by Response Surface Methodology (RSM). The effects of operating conditions (extraction time, extraction temperature and ratio of solvent to sample) on the extraction of TPC were studied using Central Composite Design (CCD). TPC was determined by Folin's Ciocalteau method and the results were expressed in (mg GAE/g Biomass). The optimal processing parameters were found to have significant effect on the extraction of TPC from Penicillium sp. A mathematical model with high determination coefficient R2 of 0.947 was obtained with optimal conditions of extraction of 5.88 minutes, 51.87 °C, and ratio of solvent to sample of 18.66:1. Under these conditions the experimental yield of TPC was 59.42 mg GAE/g Biomass which was close to the predicted value of 59.88mg GAE/g Biomass. This study indicated the potential use of MAE for extraction of antioxidants from the biomass of an endophytic fungus.