Identification, purification and functional characterization of a thermostable marine chitinase for potential fungal control via chitin degradation mechanism

Simple item page
dc.contributor.authorAtheena, P.V.
dc.contributor.authorRaval, K.
dc.contributor.authorRaval, R.
dc.date.accessioned2026-02-03T13:19:06Z
dc.date.issued2025
dc.description.abstractThe growing prevalence of treatment-resistant Candida species highlights an urgent need for innovative antifungal therapies. The current range of antifungals, limited to polyenes, azoles, and echinocandins, are becoming insufficient due to the rise of resistance, including cross-resistance among fungal strains. Marine environment is an underexplored reservoir of unique enzymes which can be extremophilic. This study presents the cloning and expression of a chitinase gene from the bacterium Bacillus thuringiensis (BtChi), expressed in an E. coli system, yielding a protein with a molecular weight of approximately 71 kDa. Disc diffusion and MIC experiments indicated that 5 ?g/mL chitinase efficiently suppressed the growth of Candida albicans. Initial characterization identified the optimal activity at 40 °C and pH 7.0. The enzyme retained over 75 % activity across a pH range of 4–8 and a temperature range of 30–70 °C after 120 min. Activity was further enhanced by 24 % with 100 mM Na+. Kinetic parameters with colloidal chitin revealed K<inf>m</inf> and V<inf>max</inf> values to be 0.05 mg/mL and 1.37 U/mL respectively. This study holds the potential of developing a potent natural anti-fungal against the present day chemical counterparts. © 2025 The Authors
dc.identifier.citationCarbohydrate Research, 2025, 558, , pp. -
dc.identifier.issn86215
dc.identifier.urihttps://doi.org/10.1016/j.carres.2025.109654
dc.identifier.urihttps://idr.nitk.ac.in/handle/123456789/19959
dc.publisherElsevier Ltd
dc.subjectBacteriology
dc.subjectChitin
dc.subjectCloning
dc.subjectDegradation
dc.subjectEnzymes
dc.subjectEscherichia coli
dc.subjectGene expression
dc.subjectYeast
dc.subjectAntifungals
dc.subjectCandida species
dc.subjectChitin degradation
dc.subjectChitinases
dc.subjectDegradation mechanism
dc.subjectExo-chitinase
dc.subjectFunctional characterization
dc.subjectGibson cloning
dc.subjectMarine
dc.subjectThermostable
dc.subjectCandida
dc.subjectPurification
dc.subjectaluminum
dc.subjectamphotericin
dc.subjectantifungal agent
dc.subjectbacterial enzyme
dc.subjectcalcium ion
dc.subjectchitin
dc.subjectchitinase
dc.subjectcobalt
dc.subjectcuprous ion
dc.subjectgenomic DNA
dc.subjectiron
dc.subjectmagnesium ion
dc.subjectmanganese
dc.subjectnatural product
dc.subjectnickel
dc.subjectpotassium ion
dc.subjectRNA 16S
dc.subjectsingle stranded DNA
dc.subjectsodium ion
dc.subjectzinc ion
dc.subjectaffinity chromatography
dc.subjectamino terminal sequence
dc.subjectantifungal activity
dc.subjectArthrobacter
dc.subjectArticle
dc.subjectBacillus thuringiensis
dc.subjectbacterial membrane
dc.subjectBotrytis cinerea
dc.subjectCandida albicans
dc.subjectcandidiasis
dc.subjectcell membrane permeability
dc.subjectchemical structure
dc.subjectcontrolled study
dc.subjectdisk diffusion
dc.subjectdrug identification
dc.subjectdrug potency
dc.subjectdrug purification
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectenzyme specificity
dc.subjectgene amplification
dc.subjectgenetic code
dc.subjecthigh performance liquid chromatography
dc.subjectkinetic parameters
dc.subjectmarine environment
dc.subjectMTT assay
dc.subjectnephrotoxicity
dc.subjectnonhuman
dc.subjectpH
dc.subjectphylogeny
dc.subjectpolymerase chain reaction
dc.subjectprocess optimization
dc.subjectprotein expression
dc.subjectprotein folding
dc.subjectquantitative analysis
dc.subjectRNA sequence
dc.subjectspectrofluorometry
dc.subjectspore germination
dc.subjectsubstrate concentration
dc.subjectsystemic mycosis
dc.subjecttemperature
dc.subjectthermostability
dc.subjectthin layer chromatography
dc.subjectWestern blotting
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectenzyme stability
dc.subjectenzymology
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectisolation and purification
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmicrobial sensitivity test
dc.subjectAntifungal Agents
dc.subjectEnzyme Stability
dc.subjectHydrogen-Ion Concentration
dc.subjectKinetics
dc.subjectMicrobial Sensitivity Tests
dc.subjectTemperature
dc.titleIdentification, purification and functional characterization of a thermostable marine chitinase for potential fungal control via chitin degradation mechanism

Files

Collections

Journal Articles