An Effective Multi-Label Protein Sub-Chloroplast Localization Prediction by Skipped-Grams of Evolutionary Profiles Using Deep Neural Network

Abstract

Chloroplast is one of the most classic organelles in algae and plant cells. Identifying the locations of chloroplast proteins in the chloroplast organelle is an important as well as a challenging task in deciphering their functions. Biological-based experiments to identify the Protein Sub-Chloroplast Localization (PSCL) is time-consuming and cost-intensive. Over the last decade, a few computational methods have been developed to predict PSCL in which earlier works assumed to predict only single-location; whereas, recent works are able to predict multiple-locations of chloroplast organelle. However, the performances of all the state-of-the-art predictors are poor. This article proposes a novel skip-gram technique to extract highly discriminating patterns from evolutionary profiles and a multi-label deep neural network to predict the PSCL. The proposed model is assessed on two publicly available datasets, i.e., Benchmark and Novel. Experimental results demonstrate that the proposed work outperforms significantly when compared to the state-of-the-art multi-label PSCL predictors. A multi-label prediction accuracy (i.e., Overall Actual Accuracy) of the proposed model is enhanced by an absolute minimum margin of 6.7 percent on Benchmark dataset and 7.9 percent on Novel dataset when compared to the best PSCL predictor from the literature. Further, result of statistical t-test concludes that the performance of the proposed work is significantly improved and thus, the proposed work is an effective computational model to solve multi-label PSCL prediction. The proposed prediction model is hosted on web-server and available at https://nitkit-vgst727-nppsa.nitk.ac.in/deeplocpred/. © 2004-2012 IEEE.