Faculty Publications
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Item Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system(Springer India sanjiv.goswami@springer.co.in, 2017) Pawar, S.S.; Iyyaswami, R.; Belur, P.D.The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in n-heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in n-heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent. © 2017, Association of Food Scientists & Technologists (India).Item Synthesis and characterization of microporous hollow core-shell silica nanoparticles (HCSNs) of tunable thickness for controlled release of doxorubicin(Springer Netherlands rbk@louisiana.edu, 2018) Deepika, D.; JagadeeshBabu, J.B.Hollow core-shell silica nanoparticles (HCSNs) are being considered as one of the most favorable drug carriers to accomplish targeted drug delivery. In the present study, we developed a simple two-step method, employing polystyrene (PS) nanoparticles (150 ± 20 nm) as a sacrificial template for the synthesis of microporous HCSNs of size 230 ± 30 nm. PS core and the wall structure directing agent cetyl trimethyl ammonium bromide (CTAB) were removed by calcination. Monodispersed spherical HCSNs were synthesized by optimising the parameters like water/ethanol volume ratio, PS/tetraethyl orthosilicate (TEOS) weight ratio, concentration of ammonia, and CTAB. Transmission electron microscopy (TEM) revealed the formation of hollow core-shell structure of silica with tunable thickness from 15 to 30 nm while tailoring the concentration of silica precursor. The results obtained from the cumulative release studies of doxorubicin loaded microporous HCSNs demonstrated the dependence of shell thickness on the controlled drug release behavior. HCSNs with highest shell thickness of 30 nm and lowest surface area of 600 m2/g showed delay in the doxorubicin release, proving their application as a drug carrier in targeted drug delivery systems. The novel concept of application of microporous HCSNs of pore size ~ 1.3 nm with large specific surface area in the field of drug delivery is successful. © 2018, Springer Nature B.V.Item Selective encapsulation of quercetin from dry onion peel crude extract in reassembled casein particles(Institution of Chemical Engineers, 2019) Ghatak, D.; Iyyaswami, R.Quercetin, a lipophilic dietary flavonoid, used as a therapeutic agent and a food component was encapsulated with casein particles to improve its water solubility as well as bioavailability in the food and pharmaceutical formulations. The nano-structured casein particles were reassembled with pure quercetin from the synthetic solution and the encapsulation yield was assessed by studying the effect of pH and the concentration of casein and additives like salts and Cetyl trimethylammonium bromide (CTAB). A maximum encapsulation yield of 97% was obtained for the reassembled casein particles formed with the addition of 0.5% (w/v) sodium caseinate, 0.1 M of calcium chloride, 0.5 M of di potassium hydrogen phosphate, 0.1 mM CTAB and 1 M of sodium citrate at a pH of 7. The identified process condition was further used to encapsulate the quercetin from the aqueous crude extract of dried onion peels. The microwave-assisted extraction was able to produce the crude extract with maximum quercetin content of 39.37 ?M per ml of the extract. Further the response surface methodology (RSM) was used to optimize the significant encapsulation variables for the maximum encapsulation yield of quercetin from aqueous crude extract. A maximum encapsulation yield of 96% was achieved at the pH 7.09 by using 2.1% (w/v) of sodium caseinate. © 2019 Institution of Chemical EngineersItem Lactoperoxidase partitioning from whey using the reverse micelles of non-ionic/ionic mixed surfactants: Improvement of back extraction(John Wiley and Sons Inc, 2022) Karanth, S.; Iyyaswami, R.The reverse micellar system formed with a mixture of ionic and non-ionic surfactants, AOT (Sodium bis[2-ethyl hexyl] sulfosuccinate)/Tween 80 in isooctane, was studied for the selective extraction of Bovine Lactoperoxidase (LP) from acid whey. The effect of pH and ionic strength of acid whey and surfactant concentrations were studied and a maximum of 86% LP was extracted from acid whey at pH 9.5 with the addition of 0.2 M KCl to the reverse micelles formed with AOT (115 mM)/Tween 80 (23 mM). The back extraction of LP was studied at different aqueous stripping phase pH, ionic strength and concentration of counter-ionic surfactant Cetyltrimethylammonium bromide (CTAB). The back extraction of 112% with 80% LP recovery was achieved when the stripping phase pH was 10.5 with 1.5 M KCl and 60 mM CTAB. The antimicrobial activity of the extracted LP showed reduction in colony-forming units of S. aureus. Novelty impact statement: The reverse micelles formed with AOT/Tween 80 surfactant mixture minimize the pH-dependent denaturation of LP and widen the pH window (7.5–9.5) for LP extraction. The back extraction of LP from the reverse micellar phase to aqueous stripping phase was improved by the addition of CTAB as counter-ionic surfactant. A purification fold of 11.26 achieved with minimal loss in activity of LP by retaining the native Antimicrobial characteristic. © 2022 Wiley Periodicals LLC.