Faculty Publications
Permanent URI for this communityhttps://idr.nitk.ac.in/handle/123456789/18736
Publications by NITK Faculty
Browse
3 results
Search Results
Item Controlled release of nutrients to mammalian cells cultured in shake flasks(2012) Hegde, S.; Pant, T.; Pradhan, K.; Badiger, M.; Gadgil, M.Though cell culture-based protein production processes are rarely carried out under batch mode of operation, cell line and initial process development operations are usually carried out in batch mode due to simplicity of operation in widely used scale down platforms like shake flasks. Nutrient feeding, if performed, is achieved by bolus addition of concentrated feed solution at different intervals, which leads to large transient increases in nutrient concentrations. One negative consequence is increased waste metabolite production. We have developed a hydrogel-based nutrient delivery system for continuous feeding of nutrients in scale down models like shake flasks without the need for manual feed additions or any additional infrastructure. Continuous delivery also enables maintaining nutrient concentrations at low levels, if desired. The authors demonstrate the use of these systems for continuous feeding of glucose and protein hydrolysate to a suspension Chinese Hamster Ovary (CHO) culture in a shake flask. Glucose feeding achieved using the glucose-loaded hydrogel resulted in a 23% higher integral viable cell density and an 89% lower lactate concentration at the end of the culture when compared with a bolus-feed of glucose. © 2011 American Institute of Chemical Engineers (AIChE).Item Production of bacterial cellulose from Komagataeibacter saccharivorans strain BC1 isolated from rotten green grapes(Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2018) Gopu, G.; Srinikethan, G.Bacterial cellulose (BC) is one of the prominent biopolymers that has been acquiring attention currently due to its distinctive properties and applications in various fields. The current work presents the isolation of Komagataeibacter saccharivorans strain BC1 isolated from rotten green grapes, followed by biochemical and genotypic characterization, which confirmed that the strain is capable of synthesizing cellulose. Further, production media was designed and certain variables such as carbon, nitrogen sources, pH, and temperature were optimized in order to obtain the maximum concentration of cellulose production. We found mannitol to be the ideal carbon source and yeast extract as the ideal nitrogen source with a highest BC dry yield of 1.81 ± 0.25 g/100 mL at pH 5.76 for a week at 30 °C.The charcterization of pellicles by FTIR spectrum depicted similar functional groups present in synthesized BC as that of the commercial cellulose. X-ray diffraction revealed that BC showed 82% crystallinity. Surface morphology of the dried pellicle was studied by SEM image which showed that the BC surface was tightly packed with thin fibers with less porosity. Hence the study demonstrates that the isolates of K.saccharivorans could be used to produce a biopolymer in a short period of time using a modified production medium. © 2018, © 2018 Taylor & Francis.Item Assessment of biocompatibility for citric acid crosslinked starch elastomeric films in cell culture applications(Nature Research, 2025) Pooja, N.; Ahmed, N.Y.; Mal, S.S.; Bharath, P.A.S.; Zhuo, G.-Y.; Noothalapati, H.; Managuli, V.; Mazumder, N.This study investigates the synthesis of potato starch elastomers reinforced with silicon dioxide (SiO2) and citric acid as a crosslinking agent to enhance their mechanical and barrier properties. Surface morphology analysis using optical microscopy revealed that pure potato starch films had uneven surfaces. However, higher SiO2 concentrations increased roughness, while citric acid crosslinked films displayed smoother surfaces overall. Water vapor transmission rates (WVTR) indicated that native starch films were highly hydrophilic, while SiO2 incorporation and citric acid crosslinking significantly reduced WVTR of 17% (30% lower than native film), enhancing the barrier properties. Tensile strength testing revealed that citric acid crosslinking increased the tensile strength by 25%, while SiO2 further reinforced the films but decreased elasticity by 15%. SiO2 had little impact on degradation rates, while citric acid crosslinking delayed microbial growth, extending film longevity by 20%. Biocompatibility assays using SiHa, HT-29, and HEK 293 cell lines revealed that the films had varying degrees of cell confluency. Films with both SiO2 and citric acid showed improved confluency (20% higher) compared to films containing only SiO2. However, citric acid alone resulted in the highest confluency (95% viability), suggesting its significant role in biocompatibility. This eco-friendly approach demonstrates substantial advancements in film properties, offering potential applications in diverse biomedical industries. © The Author(s) 2025.