Faculty Publications
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Item Tailoring solulan C24 based niosomes for transdermal delivery of donepezil: In vitro characterization, evaluation of pH sensitivity, and microneedle-assisted Ex vivo permeation studies(Editions de Sante editions.de.sante@wanadoo.fr, 2020) Nayak, A.S.; Chodisetti, S.; Gadag, S.; Nayak, U.Y.; Srinikethan, S.; Raval, K.The present investigation aims at encapsulating donepezil (DNP) in a niosomes to avert the side effects and to deliver the intact carrier across the skin barrier by modulating its physicochemical properties. The finding conclusively demonstrated that entrapment efficiency and the alteration in the niosome size are associated with the change in the span 60: cholesterol ratio, sonication, and hydration volume. The addition of 5 mM of solulan C24 to the optimized formulation (NSV5SolC24) formed stable niosomes with a mean particle size of 180.1 ± 1.83 nm and entrapment efficiency of 82.15% ± 1.54%. The cryo-SEM image and in vitro drug release profile revealed that the NSV5SolC24 is pH-sensitive. FTIR spectral analysis of NSV5SolC24 suggested that the ether and ester group in the NSV5SolC24 complex undergoes SN2 cleavage and hydrolysis at lower pH, thus enhancing DNP release. The microneedle (MN)-assisted studies with MN1200 showed a 29-fold increase in transdermal permeation of intact NSV5SolC24 against the passive method in porcine skin. The intact NSV5SolC24 carrying DNP was translocated across the skin barrier successfully at a steady flux rate of 9.89 ± 0.923 ?g/cm2/h. Nevertheless, further in vivo studies are recommended to elucidate the pH sensitivity and clinical efficacy of the prepared drug delivery system. © 2020 Elsevier B.V.Item Experimentally Induced Hyperglycemia in Prepubertal Phase Impairs Oocyte Quality and Functionality in Adult Mice(Endocrine Society, 2022) Predheepan, D.; Daddangadi, A.; Uppangala, S.; Koulmane Laxminarayana, S.L.K.; Raval, K.; Kalthur, G.; Kovaĉiĉ, B.; Kumar Adiga, S.Reproductive abnormalities in women with a history of childhood diabetes are believed to be partially attributed to hyperglycemia. Prolonged hyperglycemia can negatively affect ovarian function and fertility during reproductive life. To address this in an experimental setting, the present study used streptozotocin-induced hyperglycemic prepubertal mouse model. The impact of prolonged hyperglycemic exposure during prepubertal life on ovarian function, oocyte quality, and functional competence was assessed in adult mice. The ovarian reserve was not significantly altered; however, the in vitro maturation potential (P<0.001), mitochondrial integrity (P<0.01), and meiotic spindle assembly (P<0.05-0.001) in oocytes were significantly affected in hyperglycemic animals in comparison to control groups. The results from the study suggest that prepubertal hyperglycemia can have adverse effects on the oocyte functional competence and spindle integrity during the reproductive phase of life. Because these changes can have a significant impact on the genetic integrity and developmental potential of the embryos and fetus, the observation warrants further research both in experimental and clinical settings. © 2022 The Author(s). Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved.