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Author: search.filters.author.Kalaivani, S.Subject: search.filters.subject.Solvent extraction

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    Partitioning studies of ?-lactalbumin in environmental friendly poly (ethylene glycol) - Citrate salt aqueous two phase systems
    (2013) Kalaivani, S.; Iyyaswami, I.
    Aqueous two-phase systems (ATPS) formed by polymer and salt have been utilized to enrich the desired biomolecule into one of the phase with higher yield and purity. The eco-friendly, biodegradable poly ethylene glycol (PEG) and different citrate salts were chosen as ATPS phase components to investigate the partitioning behavior of ?-lactalbumin (?-La). System factors and process parameters such as type and concentration of salt, molecular weight and concentration of PEG, pH, temperature and the effect of additives were studied and the results are discussed in detail. PEG 1000-tri-potassium citrate system yields high partition coefficient of 20 with a better yield of 98 % in the top phase. The addition of NaCl as an additive and acidic pH lowers the yield of ?-La in the top phase. Influence of phase volume ratio (V r) on partitioning was studied and found that the partition coefficient remains almost constant along the tie line. High yield was achieved at a V r of 3.5 at the tie line length of 50.63 (%, w/w). © 2013 Springer-Verlag Berlin Heidelberg.
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    Synergistic extraction of ?-Lactalbumin and ?-Lactoglobulin from acid whey using aqueous biphasic system: Process evaluation and optimization
    (Elsevier, 2015) Kalaivani, S.; Iyyaswami, R.
    The present investigation focused on the simultaneous partitioning of ?-Lactalbumin (?-La) to the PEG rich phase and ?-Lactoglobulin (?-Lg) to the salt rich bottom phase from acid whey using polyethylene glycol 1000-trisodium citrate systems. The effect of Tie Line Length (TLL), pH, volume ratio and whey concentration on the proteins partitioning was evaluated. Further the Response Surface Methodology (RSM) was employed to analyze the synergistic and interactive effect of these variables (TLL, pH and whey loading) on the responses (recovery and purity of ?-La and ?-Lg). Optimum values of these variables were obtained for different goals through desirability based multi response optimization. A system of 54.12% (w/w) TLL with 30.6% (w/w) whey at pH 7.23 separates ?-La with 89% recovery and purity of 96% in the top phase, whereas that of ?-Lg in the bottom phase was 96% and 76% respectively. © 2015 Elsevier B.V.