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Author: search.filters.author.Kalaivani, S.Subject: search.filters.subject.Polyethylene glycols

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    Densities and viscosities of binary and ternary mixtures and aqueous two-phase system of poly(ethylene glycol) 2000 + diammonium hydrogen citrate + water at different temperatures
    (2012) Kalaivani, S.; Srikanth, C.K.; Iyyaswami, I.
    The densities and viscosities of aqueous solutions of poly(ethylene glycol) (PEG-2000) and diammonium hydrogen citrate were determined for mass fractions from 0.05 to 0.5 at different temperatures of (298.15, 303.15, 308.15, 313.15, and 318.15) K. The density data show a linear variation with mass fraction of the polymer and salt for all temperatures. The viscosity data of PEG 2000 and diammonium hydrogen citrate solutions were correlated as a function of mass fraction, using a nonlinear equation, for the five different temperatures covered in the present work. Densities and viscosities of PEG-diammonium hydrogen citrate two-phase systems have also been measured at (298.15, 303.15, 308.15, 313.15, and 318.15) K and are correlated with composition. The tie-line lengths (TLL) of the aqueous two-phase systems have also been estimated. © 2012 American Chemical Society.
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    Partitioning studies of ?-lactalbumin in environmental friendly poly (ethylene glycol) - Citrate salt aqueous two phase systems
    (2013) Kalaivani, S.; Iyyaswami, I.
    Aqueous two-phase systems (ATPS) formed by polymer and salt have been utilized to enrich the desired biomolecule into one of the phase with higher yield and purity. The eco-friendly, biodegradable poly ethylene glycol (PEG) and different citrate salts were chosen as ATPS phase components to investigate the partitioning behavior of ?-lactalbumin (?-La). System factors and process parameters such as type and concentration of salt, molecular weight and concentration of PEG, pH, temperature and the effect of additives were studied and the results are discussed in detail. PEG 1000-tri-potassium citrate system yields high partition coefficient of 20 with a better yield of 98 % in the top phase. The addition of NaCl as an additive and acidic pH lowers the yield of ?-La in the top phase. Influence of phase volume ratio (V r) on partitioning was studied and found that the partition coefficient remains almost constant along the tie line. High yield was achieved at a V r of 3.5 at the tie line length of 50.63 (%, w/w). © 2013 Springer-Verlag Berlin Heidelberg.