Faculty Publications

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Author: search.filters.author.Kalaivani, S.Has files: No

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    Densities and viscosities of binary and ternary mixtures and aqueous two-phase system of poly(ethylene glycol) 2000 + diammonium hydrogen citrate + water at different temperatures
    (2012) Kalaivani, S.; Srikanth, C.K.; Iyyaswami, I.
    The densities and viscosities of aqueous solutions of poly(ethylene glycol) (PEG-2000) and diammonium hydrogen citrate were determined for mass fractions from 0.05 to 0.5 at different temperatures of (298.15, 303.15, 308.15, 313.15, and 318.15) K. The density data show a linear variation with mass fraction of the polymer and salt for all temperatures. The viscosity data of PEG 2000 and diammonium hydrogen citrate solutions were correlated as a function of mass fraction, using a nonlinear equation, for the five different temperatures covered in the present work. Densities and viscosities of PEG-diammonium hydrogen citrate two-phase systems have also been measured at (298.15, 303.15, 308.15, 313.15, and 318.15) K and are correlated with composition. The tie-line lengths (TLL) of the aqueous two-phase systems have also been estimated. © 2012 American Chemical Society.
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    Partitioning studies of ?-lactalbumin in environmental friendly poly (ethylene glycol) - Citrate salt aqueous two phase systems
    (2013) Kalaivani, S.; Iyyaswami, I.
    Aqueous two-phase systems (ATPS) formed by polymer and salt have been utilized to enrich the desired biomolecule into one of the phase with higher yield and purity. The eco-friendly, biodegradable poly ethylene glycol (PEG) and different citrate salts were chosen as ATPS phase components to investigate the partitioning behavior of ?-lactalbumin (?-La). System factors and process parameters such as type and concentration of salt, molecular weight and concentration of PEG, pH, temperature and the effect of additives were studied and the results are discussed in detail. PEG 1000-tri-potassium citrate system yields high partition coefficient of 20 with a better yield of 98 % in the top phase. The addition of NaCl as an additive and acidic pH lowers the yield of ?-La in the top phase. Influence of phase volume ratio (V r) on partitioning was studied and found that the partition coefficient remains almost constant along the tie line. High yield was achieved at a V r of 3.5 at the tie line length of 50.63 (%, w/w). © 2013 Springer-Verlag Berlin Heidelberg.
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    Synergistic extraction of ?-Lactalbumin and ?-Lactoglobulin from acid whey using aqueous biphasic system: Process evaluation and optimization
    (Elsevier, 2015) Kalaivani, S.; Iyyaswami, R.
    The present investigation focused on the simultaneous partitioning of ?-Lactalbumin (?-La) to the PEG rich phase and ?-Lactoglobulin (?-Lg) to the salt rich bottom phase from acid whey using polyethylene glycol 1000-trisodium citrate systems. The effect of Tie Line Length (TLL), pH, volume ratio and whey concentration on the proteins partitioning was evaluated. Further the Response Surface Methodology (RSM) was employed to analyze the synergistic and interactive effect of these variables (TLL, pH and whey loading) on the responses (recovery and purity of ?-La and ?-Lg). Optimum values of these variables were obtained for different goals through desirability based multi response optimization. A system of 54.12% (w/w) TLL with 30.6% (w/w) whey at pH 7.23 separates ?-La with 89% recovery and purity of 96% in the top phase, whereas that of ?-Lg in the bottom phase was 96% and 76% respectively. © 2015 Elsevier B.V.
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    Continuous aqueous two-phase extraction of ?-lactalbumin from whey in conventional rotating disc contactor
    (Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2016) Kalaivani, S.; Iyyaswami, I.
    The differential partitioning of ?-La to the PEG-rich phase and ?-Lg to the salt-rich phase was studied in the continuous extractor, rotating disc contactor (RDC), using polyethylene glycol (PEG) 1000–trisodium citrate system. The performance of the extractor was evaluated in terms of holdup, mass transfer coefficient, extraction efficiency, and recovery by studying the operating variables such as phase velocities and rotor speed. The process conditions for the maximum dispersed phase mass transfer coefficient and recovery of ?-La was also obtained and reported. © 2016 Taylor & Francis.