Faculty Publications
Permanent URI for this communityhttps://idr.nitk.ac.in/handle/123456789/18736
Publications by NITK Faculty
Browse
6 results
Search Results
Item Role of N-vinyl-2-pyrrolidinone on the thermoresponsive behavior of PNIPAm hydrogel and its release kinetics using dye and vitamin-B12 as model drug(2014) Maheswari, B.; JagadeeshBabu, P.E.; Agarwal, M.Temperature-sensitive hydrogels hold great promise in biological applications as they can respond to changes in physiological temperature to produce a desired effect like controlled drug delivery. In this study, a series of poly(N-isopropylacrylamide-co-N-vinyl-2-pyrrolidinone) thermosensitive hydrogels were synthesized by radical copolymerization of NIPAm with 1-vinyl-2-pyrrolidinone (NVP). By altering the initial NIPAm/NVP mole ratios, copolymers were synthesized to have their own distinctive lower critical solution temperature which was established using differential scanning calorimetry. The swelling behavior of the hydrogel was analyzed gravimetrically and it was observed that reswelling rate increases with increasing NVP mole ratio. Further characterizations of the hydrogels were performed using Fourier transform infrared spectroscopy and scanning electron microscopy. Release kinetics with respect to temperature was studied using methylene blue dye solution and vitamin B12. Kinetic modeling of the release profile revealed that the release mechanism is a non-Fickian diffusion mechanism. These results suggested that this material has potential application as intelligent drug carriers. The quantities of residual monomers in the PIV4 hydrogel were determined by HPLC method, and the results show almost complete conversion. © 2013 Taylor & Francis.Item Extraction, optimization and characterization of collagen from sole fish skin(Elsevier B.V., 2018) Arumugam, G.K.S.; Sharma, D.; Mohan Balakrishnan, R.M.; JagadeeshBabu, P.E.In this study, collagen was successfully extracted from marine waste i.e. Sole fish skin, which is available in the coastal area of Mangalore, India. The extraction process was optimized using One Variable at a Time (OVAT) and Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was to achieve maximum yield and the extracted collagen was characterized. The optimal conditions to obtain highest collagen yield was determined to be, an acetic acid concentration of 0.54 M, NaCl concentration of 1.90 M, solvent/solid ratio of 8.97 ml/g and time of 32.32 h. The maximum collagen yield of 19.27 ± 0.05 mg/g of fish skin was achieved under the optimal conditions. The analysis of variance and contour plots exhibited a significant interaction of all the selected variables over collagen extraction process. The SDS-PAGE (Sodium dodecyl sulfate - polyacrylamide gel electrophoresis) analysis suggested that the extracted collagen contained three ?-chains i.e. (?1)2, ?2 (M.W. 118, 116 kDa) and one ? chain (M.W. 200 kDa) which was similar to commercially available calfskin Type I collagen. FT-IR (Fourier Transform Infrared Spectroscopy) analysis confirmed the existence of helical arrangements of collagen. SEM (Scanning electron microscopy) observation revealed that the extracted collagen was in the form of fibrils with irregular linkages. © 2018 Elsevier B.V.Item Role of graphene quantum dots synthesized through pyrolysis in the release behavior of temperature responsive poly (N,N-diethyl acrylamide) hydrogel loaded with doxorubicin(Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2018) Havanur, S.; JagadeeshBabu, P.E.We have reported the synthesis and characterization of new drug carrier using Poly (N,N-diethyl acrylamide) (PDEA) and graphene quantum dots (GQDs). PDEA is a stimuli-responsive, macroporous polymer which has the ability to respond to change in surrounding temperature and addition of GQDs will help in improving the inherent characteristics of PDEA. In this research work, PDEA hydrogels along with GQDs have been synthesized by free radical polymerization. The effect of various concentrations of GQDs on the property of PDEA hydrogel was studied. The structural analysis of synthesized hydrogels was done using Fourier transform infrared spectroscopy (FT–IR). The internal surface morphology of porous hydrogels was observed using scanning electron microscope (SEM) micrographs. From the analysis, it has been observed that the equilibrium swelling ratio (ESR) and reswelling kinetics of the hydrogel significantly increased as the GQDs content was varied. The cancer drug (an anthracycline that is used for cancer chemotherapy) Doxorubicin (DOX) release behavior was studied and found that the performance of hydrogel is dependent on hydrogel composition, time, and surrounding temperature. The cytotoxicity of GQDs incorporated PDEA hydrogels gave a significant report which supports the potential application of hydrogel as an intelligent drug carrier. © 2018, © 2018 Taylor & Francis Group, LLC.Item Synthesis and optimization of poly (N,N-diethylacrylamide) hydrogel and evaluation of its anticancer drug doxorubicin’s release behavior(Springer London, 2019) Havanur, S.; Farheenand, V.; JagadeeshBabu, P.E.A macroporous temperature-responsive poly(N,N-diethylacrylamide) (PDEA) hydrogel was synthesized and optimized through free radical polymerization. The optimized hydrogel was achieved by evaluating the swelling characteristics, physical stability and mechanical strength through altering the components namely concentration of N,N-diethylacrylamide (monomer), ammonium peroxodisulfate (initiator), N,N?-methylbisacrylamide (cross-linker) and N,N,N?,N?-tetramethylethylenediamine (accelerator). The equilibrium swelling behavior was performed gravimetrically, and the PDEA hydrogel synthesized at 36 °C exhibited a maximum swelling of 18.332 g.g ?1 . Also, the LCST of the prepared PDEA hydrogel was found to be around 29 °C. However, the results of time-controlled swelling and deswelling kinetics indicated that hydrogels are temperature sensitive. Further, characterization of the hydrogel was performed using scanning electron microscopy, differential scanning calorimetry, thermal gravimetric analysis, and Fourier transform infrared spectroscopy. The hydrogel was assessed for its cytotoxicity in MDA-MB-231 cell line by MTT assay. The release behavior of anticancer drug doxorubicin (DOX), a hydroxyl derivative of anthracycline, was studied at above and below the LCST temperature. It was found that the DOX release from the DOX-loaded hydrogels was significantly improved when the surrounding temperature of the release media was increased near to physiological temperature. The cumulative release profile of hydrogel at different temperatures was fitted to different kinetic model equations and non-Fickian diffusion release mechanism was revealed. These results suggest that PDEA has a potential application as an intelligent drug carrier. © 2018, Iran Polymer and Petrochemical Institute.Item Mechanistic insight into the endophytic fungus mediated synthesis of protein capped ZnO nanoparticles(Elsevier Ltd, 2019) Kadam, V.V.; JagadeeshBabu, P.E.; Mohan Balakrishnan, R.Present investigation is focused on the development of environment friendly protocol for ZnO nanoparticles synthesis using mycelial free filtrate of unexplored endophytic fungus Cochliobolus geniculatus. Zinc metal tolerant endophyte was isolated from leaves of Nothapodytes foetida and identified based on rDNA internal transcribed spacer region sequence. Surface plasmon resonance examined by UV–VIS spectroscopy analysis confirmed the synthesis of ZnO nanoparticles; exhibiting a band gap of 3.28 eV. The obtained nanoparticles were characterized by energy dispersive X-ray (EDX), high resolution transmission electron microscopy and XRD analysis. The synthesized ZnO nanoparticles were found to be polydispersed and well distributed deprived of agglomeration, crystalline, quasi spherical particles exhibiting a narrow range distribution. Fluorescence, FTIR and UV–VIS spectra depicted presence of extracellular proteins which was further confirmed by SDS-PAGE study that displayed occurrence of 52 kDa and 58 kDa protein bands involved in the synthesis of ZnO nanoparticles and further act as capping material. © 2019 Elsevier B.V.Item One step synthesis of silver nanowires using fructose as a reducing agent and its antibacterial and antioxidant analysis(Institute of Physics Publishing helen.craven@iop.org, 2019) Sharma, D.; Rakshana, D.A.; Mohan Balakrishnan, R.M.; JagadeeshBabu, P.E.Silver nanowires were synthesised using hydrothermal method by reducing silver nitrate (AgNO3) using fructose in the presence of poly-vinylpyrrolidone (PVP). The parameters such as the effect of process temperature, AgNO3 molarity, PVP and fructose (C6H12O6) concentration influencing the synthesis of silver nanowires (Ag NWs) were investigated. The scanning electron microscope (SEM) images showed that ultra-long, uniform and thin silver nanowires were obtained under optimized conditions; 0.02 M AgNO3, 0.016 g ml-1 of fructose, 0.16 g ml-1 of PVP at 160 °C within 22 h. The dynamic light scattering (DLS) analysis revealed that the silver nanowires obtained have an average diameter of 77 nm possessing high level of crystallinity with face centered cubic (fcc) phase that is evident from the x-ray diffraction (XRD) patterns peaked at (111), (200), (220), (311) and (222) planes. FT-IR (Fourier transform infrared spectroscopy) results suggested that there is adsorption of PVP molecules on the silver atoms. Ag NWs exhibited better antibacterial activity against Escherichia coli and high antioxidant activity against 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenger. This work gives a green approach to the hydrothermal synthesis of Ag NWs using fructose with a promising antibacterial and antioxidant properties. © 2019 IOP Publishing Ltd.