Faculty Publications

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Author: search.filters.author.JagadeeshBabu, J.B.Has files: No

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    TiO2 nanosheet incorporated polysulfone ultrafiltration membranes for dye removal
    (Desalination Publications dwt@deswater.com, 2018) Nair, A.K.; Kumar, B.V.; Kalaiarasan, G.; JagadeeshBabu, J.B.
    Incorporation of nanomaterials in polymeric membranes is an effective means to improve membrane performance. In the present work, a novel additive TiO2 nanosheet was incorporated in polysulfone membrane. TiO2 nanosheets were synthesised by hydro-thermal method and blended with polysulfone to give nanocomposite membranes. The membranes performance was evaluated via pure water flux, bovine serum albumin rejection and anti fouling studies. Further the membranes were subjected to dye rejection application using Congo red and Rhodamine-B dyes. The membranes were characterised using scanning electron microscopy; X-ray diffraction and contact angle measurement. The nanocomposite membranes exhibited superior permeation, anti fouling and dye rejection traits. © 2018 Desalination Publications. All rights reserved.
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    Synthesis and characterization of microporous hollow core-shell silica nanoparticles (HCSNs) of tunable thickness for controlled release of doxorubicin
    (Springer Netherlands rbk@louisiana.edu, 2018) Deepika, D.; JagadeeshBabu, J.B.
    Hollow core-shell silica nanoparticles (HCSNs) are being considered as one of the most favorable drug carriers to accomplish targeted drug delivery. In the present study, we developed a simple two-step method, employing polystyrene (PS) nanoparticles (150 ± 20 nm) as a sacrificial template for the synthesis of microporous HCSNs of size 230 ± 30 nm. PS core and the wall structure directing agent cetyl trimethyl ammonium bromide (CTAB) were removed by calcination. Monodispersed spherical HCSNs were synthesized by optimising the parameters like water/ethanol volume ratio, PS/tetraethyl orthosilicate (TEOS) weight ratio, concentration of ammonia, and CTAB. Transmission electron microscopy (TEM) revealed the formation of hollow core-shell structure of silica with tunable thickness from 15 to 30 nm while tailoring the concentration of silica precursor. The results obtained from the cumulative release studies of doxorubicin loaded microporous HCSNs demonstrated the dependence of shell thickness on the controlled drug release behavior. HCSNs with highest shell thickness of 30 nm and lowest surface area of 600 m2/g showed delay in the doxorubicin release, proving their application as a drug carrier in targeted drug delivery systems. The novel concept of application of microporous HCSNs of pore size ~ 1.3 nm with large specific surface area in the field of drug delivery is successful. © 2018, Springer Nature B.V.
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    In-silico epitope identification and design of Uricase mutein with reduced immunogenicity
    (Elsevier Ltd, 2020) Nelapati, A.K.; Das, B.K.; JagadeeshBabu, J.B.; Chakraborty, D.
    The clinical utilization of Uricase against gout is limited due to the immunogenicity. In the present article, we identified the antigenic determinants of Uricase and reduced their immunogenicity via in-silico mutagenesis. Multiple sequence alignment and motif analysis were carried out to identify the conserved residues in evolutionary process. Emini surface accessibility, Parker hydrophilicity, and Karplus & Schulz flexibility methods were employed to predict the linear B-cell epitopes of both Ag-Uricase and Bf-Uricase. Deimmunization approach identified T-cell epitopes and the hot spot residues. Reduced antigenic probability was obtained in case of T159W, D169C, N264W and Y203D mutations for Ag-Uricase, while S139 V, K215W, G216 F and I172 P mutations for Bf-Uricase. The binding affinity values of uric acid towards the catalytic pocket of Ag-Uricase and Bf-Uricase models were found to be -48.71 kcal/mol and -40.93 kcal/mol, respectively. This energy is further stabilized in the mutant model by -6.36 kcal/mol and -1.45 kcal/mol for Ag-Uricase and Bf-Uricase, respectively. About 100 ns molecular dynamics simulation was performed to evaluate the conformational stability of both native and mutated Uricase. Insights obtained from this study provide guidelines for experimental design of Uricase muteins with reduced antigenicity. © 2020 Elsevier Ltd
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    Comprehensive insight into the extract optimization, phytochemical profiling, and biological evaluation of the medicinal plant Nothapodytes foetida
    (Elsevier Ltd, 2022) Susanna, D.; Mohan Balakrishnan, R.M.; JagadeeshBabu, J.B.
    Nothapodytes foetida is an endemic medicinal plant belonging to the family Icacinaceae from the deciduous forests of the Western Ghats. The extract yield, nutritional and phytochemical composition, metabolite profiling of N. foetida leaf extract has been analysed, along with the assessment of its antioxidant, anti-inflammatory, anti-microbial and anti-cancer potentialities. The effect of different extracting solvents and techniques was optimized to obtain maximum extract yield, and ultrasonication-assisted extraction with aqueous methanol as the solvent was chosen. Appreciable amounts of total phenolics (67.59 mg GAE/g DW), total flavonoids (24.75 mg QCE/g DW), and total tannins (55.67 mg GAE/g DW) were detected. FT-IR spectroscopy also confirmed the presence of alcohols, phenols, alkanes, amino acids, carboxylic acids, nitro compounds and amines in the extract. Further, GC-MS analysis detected the presence of 33 volatile compounds that comprised of viminalol, α, β-amyrins, β, γ-sitosterol, 9-methoxy-camptothecin, lupeols, and various di and tri terpenes in significant quantities. 1H NMR spectra revealed well-resolved signals for flavonoids, amino acids (trp, his, tyr, phe, ala, ile, gly, gln, thr, val) and organic acids. LC-MS analysis of the methanolic N. foetida extract depicted a higher polyphenolic content followed by the ethanolic and aqueous extracts. Additionally, the aqueous methanolic extract of N. foetida exhibited significant in-vitro antioxidant, anti-inflammatory, anti-microbial activity and anti-cancer activity. The overall results of this work ascertain the potency of N. foetida in nutraceutical and biomedical applications. © 2022
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    Synthesis, Characterization and Performance Evaluation of TiO2-SnO2 photocatalyst for Removal of Toxic Hexavalent Chromium
    (Springer Science and Business Media Deutschland GmbH, 2022) Rathna, T.; JagadeeshBabu, J.B.; Ruben Sudhakar, D.R.
    Heavy metal pollution bears a substantial threat to flora, fauna, humans, and milieu. The elimination of hexavalent chromium [Cr(VI)] from polluted water is of considerable research interest from a health and environmental viewpoint. This work aims for photocatalytic reduction of Cr(VI) to Cr(III) using TiO2 and SnO2 as catalysts. The process parameters varied are catalyst dosage, pH, initial concentration of model pollutant with citric acid (CA) as a scavenger. TiO2-SnO2 (in the molar ratios 10:1, 20:1, 30:1, and 40:1)-based catalysts were synthesized using the hydrothermal method. The 40:1 catalyst showed higher photoactivity than other catalysts and could extend the optical activity in the visible light region. The complete reduction of Cr(VI) with a concentration of 15 mg/L and pH 3.0 was achieved when catalyst concentration was 0.6 g/L over a period of 30 min. The XRD (X-Ray Diffraction Spectroscopy) and ATR-FTIR (Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy) results confirm the Ti-Sn heterojunction formation, and XPS (X-ray Photoelectron Spectroscopy) analysis corroborate the presence of trivalent chromium [Cr(III)] on TiO2-SnO2 catalyst after reduction. © 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.