Faculty Publications
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Item Computational analysis of therapeutic enzyme uricase from different source organisms(Bentham Science Publishers P.O. Box 294 Bussum 1400 AG, 2020) Nelapati, A.K.; JagadeeshBabu, J.Background: Hyperuricemia and gout are the conditions, which is a response of accumulation of uric acid in the blood and urine. Uric acid is the product of purine metabolic pathway in humans. Uricase is a therapeutic enzyme that can enzymatically reduces the concentration of uric acid in serum and urine into more a soluble allantoin. Uricases are widely available in several sources like bacteria, fungi, yeast, plants and animals. Objective: The present study is aimed at elucidating the structure and physiochemical properties of uricase by insilico analysis. Methods: A total number of sixty amino acid sequences of uricase belongs to different sources were obtained from NCBI and different analysis like Multiple Sequence Alignment (MSA), homology search, phylogenetic relation, motif search, domain architecture and physiochemical properties including pI, EC, Ai, Ii, and were performed. Results: Multiple sequence alignment of all the selected protein sequences has exhibited distinct difference between bacterial, fungal, plant and animal sources based on the position-specific existence of conserved amino acid residues. The maximum homology of all the selected protein sequences is between 51-388. In singular category, homology is between 16-337 for bacterial uricase, 14-339 for fungal uricase, 12-317 for plants uricase, and 37-361 for animals uricase. The phylogenetic tree constructed based on the amino acid sequences disclosed clusters indicating that uricase is from different source. The physiochemical features revealed that the uricase amino acid residues are in between 300-338 with a molecular weight as 33-39kDa and theoretical pI ranging from 4.95-8.88. The amino acid composition results showed that valine amino acid has a high average frequency of 8.79 percentage compared to different amino acids in all analyzed species. Conclusion: In the area of bioinformatics field, this work might be informative and a stepping-stone to other researchers to get an idea about the physicochemical features, evolutionary history and structural motifs of uricase that can be widely used in biotechnological and pharmaceutical industries. Therefore, the proposed in silico analysis can be considered for protein engineering work, as well as for gout therapy. © 2020 Bentham Science Publishers.Item Ultrasonication-assisted green synthesis and characterization of gold nanoparticles from Nothapodytes foetida: An assessment of their antioxidant, antibacterial, anticancer and wound healing potential(Editions de Sante, 2023) Susanna, D.; Mohan Balakrishnan, R.M.; JagadeeshBabu, J.Nanotechnology and biogenic synthesis of metal nanoparticles using plants have attracted immense attention recently owing to their beneficial applications. Ultrasonication technique was employed for the rapid one-step phytochemical synthesis of AuNPs from the extracts of Nothapodytes foetida leaves. The N. foetida AuNPs exhibited the characteristic UV absorption peak at 524 nm. Several analytical techniques, including FESEM, TEM, XRD, FTIR, TGA, ICP and zeta potential analysis, were performed for their effective characterization. These analyses revealed highly stable (– 60.7 mV) crystalline AuNPs, majorly spherical, ranging between 5 and 30 nm. Furthermore, CCD-RSM was incorporated to determine the influence of the temperature, N. foetida leaf extract and HAuCl4 concentration on the synthesis of AuNPs. LC-MS revealed the phytocompounds that contributed to AuNP synthesis which was subsequently confirmed by TPC and TFC reduction. Potent radical scavenging ability accompanied by the antibacterial activity of the AuNPs was also evaluated. Importantly, AuNPs also reduced the cell viability of MG63 and A549 cell lines, exhibiting significant anticancer activity along with appreciable wound-healing properties. These findings suggest that these AuNPs possess tremendous biological potentialities and can be bio-prospected further for various multidisciplinary applications. © 2023 Elsevier B.V.Item TiO2-WO3 nanocube-polyaniline hierarchical membrane for efficient removal of chromium in a photocatalytic membrane reactor(John Wiley and Sons Inc, 2023) Rathna, T.; JagadeeshBabu, J.; Ruben Sudhakar, D.R.In this article, the rational design of a physically coated TiO2-WO3 nanocube-polyaniline photocatalytic membrane (hierarchical) reactor for the efficient reduction of chromium (VI) under visible light is discussed. The incorporation of TiO2-WO3 in the polyaniline (PANI) membrane provides excellent hydrophilicity and high photo-corrosion resistance and facilitates the rapid permeation of water with a flux rate of 12 L/m2 h and higher Cr (VI) reduction. The exposure of bare PANI membranes in Cr (VI) solution leads to over-oxidation of PANI membrane and eventually leads to the destruction of the membrane. The hierarchical photocatalytic coating improves the lifetime of the membrane by blocking it from deprotonation. The photocatalytic membrane could offer 79.30% Cr (VI) reduction under visible light irradiation in a photocatalytic membrane reactor. After several reduction cycles, the membrane showed good self-cleaning ability. The present work suggests that TiO2-WO3 nanocube-coated PANI hierarchical membrane is a promising approach for the reduction and removal of Cr (VI) from wastewater. © 2023 CIWEM.Item Amidated pectin and gum Arabic aldehyde-based pH-sensitive hydrogel for targeted colonic treatment(Elsevier B.V., 2025) Singh, H.; JagadeeshBabu, J.; Mohan Balakrishnan, R.In this study, a novel pH-responsive hydrogel was developed by crosslinking amidated pectin(AmPec) with oxidised gum Arabic(GAA) by hydrogen and hemiacetal bonding without the need for toxic crosslinkers for oral delivery of doxorubicin to treat colon cancer. FTIR and NMR confirmed the amidation of pectin and oxidation of Gum Arabic. FTIR confirmed the formation of hydrogen and hemiacetal bonds in the hydrogel. X-ray diffraction(XRD) spectra showed the amorphous characteristic of AmPec-GAA hydrogels compared to their polymer precursors, confirming the formation of a crosslinked hydrogel. AmPec-GAA15 hydrogel swelled around 655 %±39.90 at pH 7.4 compared to 181 %±7.94 swelling at pH 1.2 after 72 h. The release of doxorubicin also followed the same trend, with only 4.48 % ±0.89 doxorubicin release at pH 1.2, while the drug release increased to 68.10 %±3.73 at pH 7.4 after 48 h. SEM micrographs revealed the macroporous and interconnected hydrogel structure with fewer pores in the hydrogel swelled in pH 1.2 compared with pH 7.4, where more visible pores were observed, indicating the pH-sensitive behaviour of the hydrogel. Hydrogel possessed excellent thermal and mechanical stability as revealed by TGA and rheology study, which can also be explored for tissue engineering applications. MTT assay on L929 cells showed cell viability above 95.1 %±,0.0074, demonstrating hydrogels' non-toxic and biocompatible behaviour. Meanwhile, Dox-loaded hydrogel induced higher cytotoxicity against HT-29 cells than free Dox in a dose-dependent manner. Therefore, the developed hydrogel can be used as an effective oral carrier to deliver doxorubicin to colon cancer while hindering its release in the stomach and thus preventing associated toxicity. © 2025 Elsevier B.V.