Faculty Publications
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Item Aqueous two phase based selective extraction of mannose/glucose specific lectin from Indian cultivar of Pisum sativum seed(Elsevier B.V., 2019) Rashmi, B.S.; Iyyaswami, R.Pisum sativum lectin (Psl) being a high-value protein has marked its application in the biomedical and therapeutic field. Aqueous two phase extraction (ATPE) was implemented as a selective partitioning technique for the partial purification of Psl from its seeds. PEG/citrate based biodegradable aqueous two phase system (ATPS) was screened and the factors such as the type and concentration of citrate salts, molar mass and concentration of polyethylene glycol (PEG), tie line length (TLL) and additive (NaCl) concentration, pH, crude load and volume ratio were studied for the selective partition of Psl. The Psl was successfully extracted to the top phase in the ATPS formed with 18% PEG 6000/16% sodium citrate at 41.01% TLL, 2% NaCl and pH of 7.5. A volume ratio of 0.76 and a crude load of 20% showed maximum activity yield of 122.12% with the purification factor of 16.26. The subunits of Psl namely ? and ? were identified with a molecular weight of 6 and 18 kDa respectively during the purity analysis using SDS PAGE and HPLC. © 2019 Elsevier B.V.Item Aqueous two-phase based simultaneous extraction and purification of cephalopod melanin(Taylor and Francis Ltd., 2025) Patrick, S.M.; Iyyaswami, R.; Belur, P.D.The cephalopod processing industry generates large quantities of ink sacs, an underutilized yet valuable source of eumelanin. Conventional extraction of melanin, relying on acid or alkali treatments, often leaves residual proteins and carbohydrates, requires multiple purification steps, and risks pigment integrity. This study investigated a polymer-salt-based aqueous two-phase system (ATPS) as an eco-friendly alternative for the selective extraction and purification of melanin. Screening of polyethylene glycols of varying molecular weights with inorganic salts identified the PEG 6000-K2HPO4 system as the most effective. The equilibrium characteristic of the system was assessed using Othmer-Tobias and Bancroft correlations, while the partitioning behavior of melanin was evaluated by considering the process parameters, including polymer and salt concentrations, tie-line length, volume ratio, and sample loading. Under optimal conditions (tie-line length of 53.23% (w/w), a volume ratio of 0.14, and a sample loading of 15% (w/w)), melanin recovery reached 99.1% with 95% purity in the PEG-rich phase. These results outperform conventional methods in both yield and product quality, highlighting ATPS as a sustainable and scalable strategy for valorizing cephalopod waste, with promising applications in food, pharmaceutical, and biomedicine. © 2025 Taylor & Francis Group, LLC.