Faculty Publications
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Item Continuous aqueous two-phase extraction of ?-lactalbumin from whey in conventional rotating disc contactor(Taylor and Francis Inc. 325 Chestnut St, Suite 800 Philadelphia PA 19106, 2016) Kalaivani, S.; Iyyaswami, I.The differential partitioning of ?-La to the PEG-rich phase and ?-Lg to the salt-rich phase was studied in the continuous extractor, rotating disc contactor (RDC), using polyethylene glycol (PEG) 1000–trisodium citrate system. The performance of the extractor was evaluated in terms of holdup, mass transfer coefficient, extraction efficiency, and recovery by studying the operating variables such as phase velocities and rotor speed. The process conditions for the maximum dispersed phase mass transfer coefficient and recovery of ?-La was also obtained and reported. © 2016 Taylor & Francis.Item Analysis of ionic and nonionic surfactants blends used for the reverse micellar extraction of Lactoperoxidase from whey(John Wiley and Sons Ltd, 2021) Karanth, S.; Iyyaswami, I.Bovine Lactoperoxidase (LP), a minor whey protein, is used as an antimicrobial in cosmetic, food, and pharmaceutical preparations. Industries are in pursuit of reliable, cheap, and scalable purification methods as the conventional techniques for LP purification like chromatography and membrane separation suffer from several drawbacks. The present work investigates the selective reverse micellar extraction of LP using the reverse micellar system formed by mixing food grade nonionic (Tween, Span, and Triton series) and ionic (AOT) surfactant blends. The analysis of LP extraction efficiency was performed by varying the concentration of nonionic surfactants with a constant AOT concentration of 100 mM and the initial pH of the system. Complete LP solubilization was achieved with reverse micelles formed by 100 mM AOT and 20 mM Tween 80 at pH 8. It was found that the extraction efficiency was dependent on the chain length or the number of ethylene oxide units in the Triton surfactant tail and the carbon–carbon double bond in Tween 80 tail, that is, on oleic acid. Span series however showed poor extraction in the organic phase substantiating the lesser water content. The forward extracted LP was successfully back-extracted into a fresh aqueous phase containing 1 M KCl at pH 10.5. The aqueous phase (whey) from the forward transfer can be further used to fractionate other whey proteins. © 2020 Curtin University and John Wiley & Sons, Ltd.Item Mixed Surfactant-Based Reverse Micellar Extraction Studies of Bovine Lactoperoxidase(John Wiley and Sons Inc, 2021) Karanth, S.; Iyyaswami, I.The suitability of reverse micellar extraction for recovery of bovine lactoperoxidase (LP) from aqueous solution was evaluated using systems formed by ionic and nonionic surfactant mixtures. The influence of ionic surfactant concentration, organic solvent, and pH on the extraction of LP into the reverse micellar phase was studied. The Tween® series surfactants with Aerosol-OT (bis-(2-ethylhexyl) sulfosuccinate) showed better extraction of LP in the reverse micelles (RM) compared to the Triton® and Span® series of surfactants. Complete extraction of LP from an aqueous phase of initial concentration 25 mg L?1 occurred with the RM formed by 90 mM Aerosol-OT/8 mM Tween® 80 in isooctane. The optimal pH, ionic strength, and positively charged ionic surfactant concentration for back extraction were also studied and a maximum of 95.5% back extraction efficiency and 66% LP activity recovery was obtained for a pH of 10.5,1 M KCl and 60 mM cetyltrimethylammonium bromide system. © 2021 AOCS