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    Partitioning studies of glutaminase in polyethylene glycol and salt-based aqueous two-phase systems
    (2013) Bolar, S.; Belur, P.D.; Iyyaswami, I.
    The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.