Browsing by Author "Sharma, D."

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Extraction, optimization and characterization of collagen from sole fish skin
(2018) Arumugam, G.K.S.; Sharma, D.; Raj Mohan, Balakrishnan; Ettiyappan, J.B.P.
In this study, collagen was successfully extracted from marine waste i.e. Sole fish skin, which is available in the coastal area of Mangalore, India. The extraction process was optimized using One Variable at a Time (OVAT) and Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was to achieve maximum yield and the extracted collagen was characterized. The optimal conditions to obtain highest collagen yield was determined to be, an acetic acid concentration of 0.54 M, NaCl concentration of 1.90 M, solvent/solid ratio of 8.97 ml/g and time of 32.32 h. The maximum collagen yield of 19.27 0.05 mg/g of fish skin was achieved under the optimal conditions. The analysis of variance and contour plots exhibited a significant interaction of all the selected variables over collagen extraction process. The SDS-PAGE (Sodium dodecyl sulfate - polyacrylamide gel electrophoresis) analysis suggested that the extracted collagen contained three ?-chains i.e. (?1)2, ?2 (M.W. 118, 116 kDa) and one ? chain (M.W. 200 kDa) which was similar to commercially available calfskin Type I collagen. FT-IR (Fourier Transform Infrared Spectroscopy) analysis confirmed the existence of helical arrangements of collagen. SEM (Scanning electron microscopy) observation revealed that the extracted collagen was in the form of fibrils with irregular linkages. 2018 Elsevier B.V.
Extraction, optimization and characterization of collagen from sole fish skin
(Elsevier B.V., 2018) Arumugam, G.K.S.; Sharma, D.; Mohan Balakrishnan, R.M.; JagadeeshBabu, P.E.
In this study, collagen was successfully extracted from marine waste i.e. Sole fish skin, which is available in the coastal area of Mangalore, India. The extraction process was optimized using One Variable at a Time (OVAT) and Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was to achieve maximum yield and the extracted collagen was characterized. The optimal conditions to obtain highest collagen yield was determined to be, an acetic acid concentration of 0.54 M, NaCl concentration of 1.90 M, solvent/solid ratio of 8.97 ml/g and time of 32.32 h. The maximum collagen yield of 19.27 ± 0.05 mg/g of fish skin was achieved under the optimal conditions. The analysis of variance and contour plots exhibited a significant interaction of all the selected variables over collagen extraction process. The SDS-PAGE (Sodium dodecyl sulfate - polyacrylamide gel electrophoresis) analysis suggested that the extracted collagen contained three ?-chains i.e. (?1)2, ?2 (M.W. 118, 116 kDa) and one ? chain (M.W. 200 kDa) which was similar to commercially available calfskin Type I collagen. FT-IR (Fourier Transform Infrared Spectroscopy) analysis confirmed the existence of helical arrangements of collagen. SEM (Scanning electron microscopy) observation revealed that the extracted collagen was in the form of fibrils with irregular linkages. © 2018 Elsevier B.V.
One step synthesis of silver nanowires using fructose as a reducing agent and its antibacterial and antioxidant analysis
(2019) Sharma, D.; Rakshana, D.A.; Raj Mohan, Balakrishnan; Jagadeeshbabu, P.E.
Silver nanowires were synthesised using hydrothermal method by reducing silver nitrate (AgNO3) using fructose in the presence of poly-vinylpyrrolidone (PVP). The parameters such as the effect of process temperature, AgNO3 molarity, PVP and fructose (C6H12O6) concentration influencing the synthesis of silver nanowires (Ag NWs) were investigated. The scanning electron microscope (SEM) images showed that ultra-long, uniform and thin silver nanowires were obtained under optimized conditions; 0.02 M AgNO3, 0.016 g ml-1 of fructose, 0.16 g ml-1 of PVP at 160 C within 22 h. The dynamic light scattering (DLS) analysis revealed that the silver nanowires obtained have an average diameter of 77 nm possessing high level of crystallinity with face centered cubic (fcc) phase that is evident from the x-ray diffraction (XRD) patterns peaked at (111), (200), (220), (311) and (222) planes. FT-IR (Fourier transform infrared spectroscopy) results suggested that there is adsorption of PVP molecules on the silver atoms. Ag NWs exhibited better antibacterial activity against Escherichia coli and high antioxidant activity against 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenger. This work gives a green approach to the hydrothermal synthesis of Ag NWs using fructose with a promising antibacterial and antioxidant properties. 2019 IOP Publishing Ltd.
One step synthesis of silver nanowires using fructose as a reducing agent and its antibacterial and antioxidant analysis
(Institute of Physics Publishing helen.craven@iop.org, 2019) Sharma, D.; Rakshana, D.A.; Mohan Balakrishnan, R.M.; JagadeeshBabu, P.E.
Silver nanowires were synthesised using hydrothermal method by reducing silver nitrate (AgNO3) using fructose in the presence of poly-vinylpyrrolidone (PVP). The parameters such as the effect of process temperature, AgNO3 molarity, PVP and fructose (C6H12O6) concentration influencing the synthesis of silver nanowires (Ag NWs) were investigated. The scanning electron microscope (SEM) images showed that ultra-long, uniform and thin silver nanowires were obtained under optimized conditions; 0.02 M AgNO3, 0.016 g ml-1 of fructose, 0.16 g ml-1 of PVP at 160 °C within 22 h. The dynamic light scattering (DLS) analysis revealed that the silver nanowires obtained have an average diameter of 77 nm possessing high level of crystallinity with face centered cubic (fcc) phase that is evident from the x-ray diffraction (XRD) patterns peaked at (111), (200), (220), (311) and (222) planes. FT-IR (Fourier transform infrared spectroscopy) results suggested that there is adsorption of PVP molecules on the silver atoms. Ag NWs exhibited better antibacterial activity against Escherichia coli and high antioxidant activity against 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenger. This work gives a green approach to the hydrothermal synthesis of Ag NWs using fructose with a promising antibacterial and antioxidant properties. © 2019 IOP Publishing Ltd.