Browsing by Author "Poojary, M.M."

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Extraction, characterization and biological studies of phytochemicals from Mammea suriga
(2015) Poojary, M.M.; Vishnumurthy, K.A.; Vasudeva, Adhikari, A.
Abstract The present work involves extraction of phytochemicals from the root bark of a well-known Indian traditional medicinal plant, viz. Mammea suriga, with various solvents and evaluation of their in vitro antimicrobial and antioxidant activities using standard methods. The phytochemical analysis indicates the presence of some interesting secondary metabolites like flavonoids, cardiac glycosides, alkaloids, saponins and tannins in the extracts. Also, the solvent extracts displayed promising antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and Cryptococcus neoformans with inhibition zone in a range of 20-33 mm. Further, results of their antioxidant screening revealed that aqueous extract (with IC50 values of 111.51 1.03 and 31.05 0.92 ?g/mL in total reducing power assay and DPHH radical scavenging assay, respectively) and ethanolic extract (with IC50 values of 128.00 1.01 and 33.25 0.89 ?g/mL in total reducing power assay and DPHH radical scavenging assay, respectively) were better antioxidants than standard ascorbic acid. Interestingly, FT-IR analysis of each extract established the presence of various biologically active functional groups in it. 2015 Xi'an Jiaotong University.
Extraction, characterization and biological studies of phytochemicals from Mammea suriga
(Xi'an Jiaotong University, 2015) Poojary, M.M.; Vishnumurthy, K.A.; Vasudeva Adhikari, A.V.
Abstract The present work involves extraction of phytochemicals from the root bark of a well-known Indian traditional medicinal plant, viz. Mammea suriga, with various solvents and evaluation of their in vitro antimicrobial and antioxidant activities using standard methods. The phytochemical analysis indicates the presence of some interesting secondary metabolites like flavonoids, cardiac glycosides, alkaloids, saponins and tannins in the extracts. Also, the solvent extracts displayed promising antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and Cryptococcus neoformans with inhibition zone in a range of 20-33 mm. Further, results of their antioxidant screening revealed that aqueous extract (with IC50 values of 111.51±1.03 and 31.05±0.92 ?g/mL in total reducing power assay and DPHH radical scavenging assay, respectively) and ethanolic extract (with IC50 values of 128.00±1.01 and 33.25±0.89 ?g/mL in total reducing power assay and DPHH radical scavenging assay, respectively) were better antioxidants than standard ascorbic acid. Interestingly, FT-IR analysis of each extract established the presence of various biologically active functional groups in it. © 2015 Xi'an Jiaotong University.
Green Synthesis of Silver and Gold Nanoparticles Using Root Bark Extract of Mammea suriga: Characterization, Process Optimization, and Their Antibacterial Activity
(2016) Poojary, M.M.; Passamonti, P.; Adhikari, A.V.
The present study reports the green synthesis of silver and gold nanoparticles (NPs) from their respective precursors AgNO3 and HAuCl4, using root bark extract of Mammea suriga. Further, it describes the influence of various reaction parameters, such as pH, temperature, precursor concentration, and volume of the extract, on the morphology and size of the newly synthesized NPs. The biosynthesized NPs were characterized using UV Vis spectroscopy, SEM, EDX, XRD, and FTIR. The formation of Ag and Au NPs was confirmed by their UV Vis spectra. Ag NPs were efficiently synthesized at pH 10, with precursor concentration of 1 mM of AgNO3 and a reaction temperature of 80 C, while Au NPs were successfully obtained at pH 8, with precursor concentration of either 1 or 3 mM HAuCl4, and the reaction was maintained at room temperature. The SEM study revealed that the particle size decreases with an increase in the extract volume used in the reaction. The XRD analysis confirmed the formation of metallic Ag and Au NPs having an average size of 50 and 22 nm, respectively. Further, the FTIR spectral data established the role of various functional groups of biomolecules involved in bioreduction as well as capping of NPs. The in vitro antibacterial screening results indicated that the NPs are potential antibacterial agents. Conclusively, the overall study showed that the root bark extract of M. suriga is an excellent eco-friendly and non-toxic source for the synthesis of biologically active Ag and Au NPs at optimal conditions. 2016, Springer Science+Business Media New York.
Green Synthesis of Silver and Gold Nanoparticles Using Root Bark Extract of Mammea suriga: Characterization, Process Optimization, and Their Antibacterial Activity
(Springer New York LLC barbara.b.bertram@gsk.com, 2016) Poojary, M.M.; Passamonti, P.; Vasudeva Adhikari, A.V.
The present study reports the green synthesis of silver and gold nanoparticles (NPs) from their respective precursors AgNO3 and HAuCl4, using root bark extract of Mammea suriga. Further, it describes the influence of various reaction parameters, such as pH, temperature, precursor concentration, and volume of the extract, on the morphology and size of the newly synthesized NPs. The biosynthesized NPs were characterized using UV–Vis spectroscopy, SEM, EDX, XRD, and FTIR. The formation of Ag and Au NPs was confirmed by their UV–Vis spectra. Ag NPs were efficiently synthesized at pH 10, with precursor concentration of 1 mM of AgNO3 and a reaction temperature of 80 °C, while Au NPs were successfully obtained at pH 8, with precursor concentration of either 1 or 3 mM HAuCl4, and the reaction was maintained at room temperature. The SEM study revealed that the particle size decreases with an increase in the extract volume used in the reaction. The XRD analysis confirmed the formation of metallic Ag and Au NPs having an average size of 50 and 22 nm, respectively. Further, the FTIR spectral data established the role of various functional groups of biomolecules involved in bioreduction as well as capping of NPs. The in vitro antibacterial screening results indicated that the NPs are potential antibacterial agents. Conclusively, the overall study showed that the root bark extract of M. suriga is an excellent eco-friendly and non-toxic source for the synthesis of biologically active Ag and Au NPs at optimal conditions. © 2016, Springer Science+Business Media New York.