Browsing by Author "Mutalik, S."

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Preparation of gold nanoparticles by novel bacterial exopolysaccharide for antibiotic delivery
(2016) Pradeepa; Vidya, S.M.; Mutalik, S.; Udaya, Bhat, K.; Huilgol, P.; Avadhani, K.
Aim To develop potent drug delivery agents to treat multidrug resistant (MDR) bacterial infections using gold nanoparticles (AuNPs) prepared by bacterial exopolysaccharide (EPS) being a reducing and stabilizing agent. Methods Gold nanoparticles were prepared by EPS, extracted from Lactobacillus plantarum. AuNPs were characterized by UV-visible spectrophotometer, transmission electron microscope (TEM), zeta potential and Fourier transform infrared spectroscopy. Antibiotics used for functionalization of AuNPs were levofloxacin, cefotaxime, ceftriaxone and ciprofloxacin. The resulted functionalized AuNPs were tested against various MDR bacteria by employing different assays such as well diffusion assay, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time killing assay. Key findings The nanostructures exhibited excellent bactericidal activity and reduced MIC and MBC against MDR Gram positive and Gram negative bacteria compared to free drugs. Escherichia coli was the most susceptible MDR bacteria followed by Klebsiella pneumoniae and Staphylococcus aureus. TEM results revealed that the bactericidal activity of nanostructures could be mediated by penetration, loss of cytoplasmic contents and cell lysis. Significance Antibiotic functionalized nanostructures were more potent than free drugs and could be used as potent drug delivery vehicles. 2016 Elsevier Inc. All rights reserved.
Preparation of gold nanoparticles by novel bacterial exopolysaccharide for antibiotic delivery
(Elsevier Inc. usjcs@elsevier.com, 2016) Pradeepa, n.; Vidya, S.M.; Mutalik, S.; Bhat, K.U.; Huilgol, P.; Avadhani, K.
Aim To develop potent drug delivery agents to treat multidrug resistant (MDR) bacterial infections using gold nanoparticles (AuNPs) prepared by bacterial exopolysaccharide (EPS) being a reducing and stabilizing agent. Methods Gold nanoparticles were prepared by EPS, extracted from Lactobacillus plantarum. AuNPs were characterized by UV-visible spectrophotometer, transmission electron microscope (TEM), zeta potential and Fourier transform infrared spectroscopy. Antibiotics used for functionalization of AuNPs were levofloxacin, cefotaxime, ceftriaxone and ciprofloxacin. The resulted functionalized AuNPs were tested against various MDR bacteria by employing different assays such as well diffusion assay, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time killing assay. Key findings The nanostructures exhibited excellent bactericidal activity and reduced MIC and MBC against MDR Gram positive and Gram negative bacteria compared to free drugs. Escherichia coli was the most susceptible MDR bacteria followed by Klebsiella pneumoniae and Staphylococcus aureus. TEM results revealed that the bactericidal activity of nanostructures could be mediated by penetration, loss of cytoplasmic contents and cell lysis. Significance Antibiotic functionalized nanostructures were more potent than free drugs and could be used as potent drug delivery vehicles. © 2016 Elsevier Inc. All rights reserved.
Stimuli-responsive and cellular targeted nanoplatforms for multimodal therapy of skin cancer
(Elsevier B.V., 2021) Padya, B.S.; Pandey, A.; Pisay, M.; Koteshwara, K.B.; Raghu Chandrashekar, R.; Bhat, K.U.; Biswas, S.; Mutalik, S.
Interdisciplinary applications of nanopharmaceutical sciences have tremendous potential for enhancing pharmacokinetics, efficacy and safety of cancer therapy. The limitations of conventional therapeutic platforms used for skin cancer therapy have been largely overcome by the use of nanoplatforms. This review discusses various nanotechnological approaches experimented for the treatment of skin cancer. The review describes various polymeric, lipidic and inorganic nanoplatforms for efficient therapy of skin cancer. The stimuli-responsive nanoplatforms such as pH-responsive as well as temperature-responsive platforms have also been reviewed. Different strategies for potentiating the nanoparticles application for cancer therapy such as surface engineering, conjugation with drugs, stimulus-responsive and multimodal effect have also been discussed and compared with the available conventional treatments. Although, nanopharmaceuticals face challenges such as toxicity, cost and scale-up, efforts put-in to improve these drawbacks with continuous research would deliver exciting and promising results in coming days. © 2020 Elsevier B.V.